| Detailed Information for Phage Wolfstar |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-24275 |
| Found By | Felix Lady |
| Year Found | 2023 |
| Location Found | Cullowhee, NC United States |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.310231 N, 83.187555 W Map |
| Discovery Notes | Found under a bridge at a river bank in very wet soil. |
| Naming Notes | Was originally going to be named Nyx but phagesdb does not allow 3 letter names. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1045 |
| Genome length (bp) | 64887 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3746 bp |
| GC Content | 63.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | ED |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Number of Genes | 121 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ114749 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5*10e8 PFU/mL |
| Pitt Freezer Box# |
175 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
