| Detailed Information for Phage Wrigley | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kip Cullinan and Kaarina Marttila |
| Year Found | 2021 |
| Location Found | Johnston, IA United States |
| Finding Institution | Northwestern College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 41.673144 N, 93.725921 W Map |
| Discovery Notes | This virus was discovered in a grassy region near a creek with a large amount of vegetation. The sample was obtained approximately 9 inches below the ground, and the area received a small amount of precipitation the previous evening. The soil was clumped, moist, and contained organic matter. |
| Naming Notes | This phage is named after my (Kip's) dog, Wrigley. As a life long Chicago Cubs fan, Wrigley was named after their stadium, Wrigley Field. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1445 |
| Genome length (bp) | 51878 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CY |
| Subcluster | CY4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Northwestern College |
| Annotation Status | In GenBank |
| Plaque Notes | Wrigley produces medium sized, circular plaques that have distinct edges. In other words, the edges of Wrigley do not gradually fade into the bacteria; it has a definitive line of where it stops. Furthermore, Wrigley produces gray, hazy mesas that appear in the middle of the plaque, which is especially notable at high concentrations of the virus. |
| Morphotype | Siphoviridae |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewWrigley_1 Wrigley_2 Wrigley_3 Wrigley_4 Wrigley_5 Wrigley_6 Wrigley_7 Wrigley_8 Wrigley_9 Wrigley_10 Wrigley_11 Wrigley_12 Wrigley_13 Wrigley_14 Wrigley_15 Wrigley_16 Wrigley_17 Wrigley_18 Wrigley_19 Wrigley_20 Wrigley_21 Wrigley_22 Wrigley_23 Wrigley_24 Wrigley_25 Wrigley_26 Wrigley_27 Wrigley_28 Wrigley_29 Wrigley_30 Wrigley_31 Wrigley_32 Wrigley_33 Wrigley_34 Wrigley_35 Wrigley_36 Wrigley_37 Wrigley_38 Wrigley_39 Wrigley_40 Wrigley_41 Wrigley_42 Wrigley_43 Wrigley_44 Wrigley_45 Wrigley_46 Wrigley_47 Wrigley_48 Wrigley_49 Wrigley_50 Wrigley_51 Wrigley_52 Wrigley_53 Wrigley_54 Wrigley_55 Wrigley_56 Wrigley_57 Wrigley_58 Wrigley_59 Wrigley_60 Wrigley_61 Wrigley_62 Wrigley_63 Wrigley_64 Wrigley_65 Wrigley_66 Wrigley_67 Wrigley_68 Wrigley_69 Wrigley_70 Wrigley_71 Wrigley_72 Wrigley_73 Wrigley_74 Wrigley_75 Wrigley_76 Wrigley_77 Wrigley_78 Wrigley_79 Wrigley_80 Wrigley_81 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON108651 |
| Refseq Number | None yet |
| Published in Paper | Bastian et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Microbacterium Phages Clayda5 and Gshelby23 and Gordonia Phages Wrigley and Santhid |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 137 |
| Pitt Freezer Box Grid# | B4 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
