| Detailed Information for Phage Azathoth | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Hunter Angle |
| Year Found | 2017 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 18°C |
| GPS Coordinates | 40.086221 N, 75.229467 W Map |
| Discovery Notes | Found just under a stump of a tree, about 65 degrees F (18.3 C) when found. Lightly rained early that morning. |
| Naming Notes | I'm an HP Lovecraft fan. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 13794 |
| Genome length (bp) | 15556 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotation Status | In GenBank |
| Plaque Notes | Azathoth's plaque morphology was very inconsistent. The size of the plaques varied greatly from being very small, pin prick plaques, to much larger plaques. I tried to streak the two sizes out many times, but I could never get any separation. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAzathoth_1 Azathoth_2 Azathoth_3 Azathoth_4 Azathoth_5 Azathoth_6 Azathoth_7 Azathoth_8 Azathoth_9 Azathoth_10 Azathoth_11 Azathoth_12 Azathoth_13 Azathoth_14 Azathoth_15 Azathoth_16 Azathoth_17 Azathoth_18 Azathoth_19 Azathoth_20 Azathoth_21 Azathoth_22 Azathoth_23 Azathoth_24 Azathoth_25 Azathoth_26 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH576948 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1*10^8 |
| Date of SEA Lysate Titering | Jun 12, 2017 |
| Pitt Freezer Box# | 68 |
| Pitt Freezer Box Grid# | C11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
