| Detailed Information for Phage Azathoth | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Hunter Angle |
| Year Found | 2017 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 18°C |
| GPS Coordinates | 40.086221 N, 75.229467 W Map |
| Discovery Notes | Found just under a stump of a tree, about 65 degrees F (18.3 C) when found. Lightly rained early that morning. |
| Naming Notes | I'm an HP Lovecraft fan. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 13794 |
| Genome length (bp) | 15556 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewArby Azathoth Blair CGermain Chestnut Copper Courtney3 Decurro Dewayne Elkhorn Guntur Hunnie Inspire2 Jessica Kels KylieMac Laila Link Lore LouisXIV Maggie Mariposa Massimo Moloch Muttlie Prospero Ronnie Sandman Saphira Seume Sourignavong StewieGriff Stratus Swenson Taj14 TinoCrisci Toulouse TymAbreu Yank |
| Annotating Institution | Cabrini University |
| Annotation Status | In GenBank |
| Plaque Notes | Azathoth's plaque morphology was very inconsistent. The size of the plaques varied greatly from being very small, pin prick plaques, to much larger plaques. I tried to streak the two sizes out many times, but I could never get any separation. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAzathoth_1 Azathoth_2 Azathoth_3 Azathoth_4 Azathoth_5 Azathoth_6 Azathoth_7 Azathoth_8 Azathoth_9 Azathoth_10 Azathoth_11 Azathoth_12 Azathoth_13 Azathoth_14 Azathoth_15 Azathoth_16 Azathoth_17 Azathoth_18 Azathoth_19 Azathoth_20 Azathoth_21 Azathoth_22 Azathoth_23 Azathoth_24 Azathoth_25 Azathoth_26 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH576948 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1*10^8 |
| Date of SEA Lysate Titering | Jun 12, 2017 |
| Pitt Freezer Box# | 68 |
| Pitt Freezer Box Grid# | C11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
