Arthrobacter phage Chestnut
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Detailed Information for Phage Chestnut
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByAndrew Conboy
Year Found2015
Location FoundRadnor, PA USA
Finding InstitutionCabrini University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.099299 N, 75.259635 W Map
Discovery NotesFrom McCarthy Park Stormwater Retention Basin in Whitemarsh Township, PA. Soil contained decomposing organic material, as well as a high amount of moisture from a nearby pond. From the same soil sample as Conboy phage.
Naming NotesChestnut was named after my home institution, Chestnut Hill College, in Philadelphia, Pennsylvania.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 16, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3314
Genome length (bp)15556
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCGCGCCACC
GC Content60.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPhage tends to form mostly large plaques, with the occasional smaller sized plaque. Optimal temperature is between room temperature and 30 degrees Celsius. Does not grow at 37 degrees Celsius. Grows just as well without any calcium (at optimal temperature) as it does with 25 mM CaCl2 concentration.
Number of Genes25
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY434670
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator Cabrini College
SEA Lysate Titer 1.26E10
Date of SEA Lysate Titering Jul 15, 2015
Pitt Freezer Box# 2
Pitt Freezer Box Grid# E5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload