| Detailed Information for Phage Battuta |
| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Hamza Kayani |
| Year Found | 2019 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.12311 N, 97.08537 W Map |
| Discovery Notes | The soil sample was collected on August 26th, 2019. Outside the Business Leadership Building on the UNT campus. The soil was collected about an inch deep. It was medium brown and contained some roots. The outside temperature was 86 degrees F.
The soil was donated to me by Marilyn Jimenez |
| Naming Notes | Battuta is the name of a famous Arabian historian and traveler who documented much of Eurasian and mongol history during the middle ages. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 390 |
| Genome length (bp) | 130734 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12579 bp |
| GC Content | 49.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | I did not need to perform a lysogeny test. No Mesa was observed in spot test after re-incubation. Leads us to believe that the phage is not a temperate phage. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are all 0.3-0.5 mm in diameter. Clear. Round. |
| Morphotype | Siphoviridae |
| Number of Genes | 239 |
| Number of tRNAs | 43 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW507134 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6x10^8 pfu/ml |
| Pitt Freezer Box# |
104 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
