The Actinobacteriophage Database

Streptomyces phage Braelyn

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(S. griseus NBRC13350)

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(S. scabiei 82-22)

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Detailed Information for Phage Braelyn
Discovery Information
Isolation Host	Streptomyces griseus ATCC 10137
Found By	Sarah Kempf
Year Found	2018
Location Found	Greenwood, IN USA
Finding Institution	University of Evansville
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	39.612021 N, 86.214723 W Map
Discovery Notes	Physical characteristics of collection: Damp, dark brown, full of organic matter. Collected from flower garden, approximately 2 inches under surface. The last rainfall was 36 hours prior to collection, rainfall was moderate for approximately 45 minutes. Ambient temperature: 92 degrees Fahrenheit
Naming Notes	My cousin's name is Braelyn
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 10, 2019
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	601
Genome length (bp)	131234
Character of genome ends	Direct Terminal Repeat
Direct Terminal Repeat Length	10824 bp
GC Content	50.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BE
Subcluster	BE1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Anedea (BE1) Angela (BE1) Bartholomune (BE1) BlueOtter (BE1) Bmoc (BE1) Braelyn (BE1) Cadmus (BE1) Coogler (BE1) Cross (BE1) Cursive (BE1) Daubenski (BE1) Davielle (BE1) EGole (BE1) Evy (BE1) HangryHippo (BE1) Jay2Jay (BE1) Larnav (BE1) Leo04 (BE1) Liandry (BE1) LilMartin (BE1) Lululemon (BE1) Marsus (BE1) Mildred21 (BE1) MulchMansion (BE1) Navo (BE1) NootNoot (BE1) PacManQ (BE1) Paradiddles (BE1) Peebs (BE1) Pepperwood (BE1) Persimmon (BE1) PinkiePie (BE1) Riptide (BE1) Samisti12 (BE1) Scheme (BE1) Shuckle (BE1) Squillium (BE1) Sushi23 (BE1) Targaryen (BE1) Teutsch (BE1) Tribute (BE1) Warpy (BE1) Watermoore (BE1) WhereRU (BE1) Amabiko (BE2) AngryGiraffe (BE2) Battuta (BE2) Birchlyn (BE2) BoomerJR (BE2) Bordeaux (BE2) CeilingFan (BE2) Elmer (BE2) Enygma (BE2) Genie2 (BE2) Gibbi (BE2) IchabodCrane (BE2) JimJam (BE2) Jollison (BE2) Karimac (BE2) KentuckyRacer (BE2) LukeCage (BE2) MindFlayer (BE2) Mugiwara (BE2) PumpkinSpice (BE2) Quaran19 (BE2) Rikishi (BE2) SaltySpitoon (BE2) Sollertia (BE2) Spelly (BE2) Spilled (BE2) Stanimal (BE2) Starbow (BE2) StarPlatinum (BE2) Tomas (BE2) TomSawyer (BE2) Wipeout (BE2) Wofford (BE2) Yaboi (BE2)
Annotating Institution	University of Evansville
Annotation Status	In GenBank
Plaque Notes	Tiny to small plaques, not cloudy, well defined
Morphotype	Siphoviridae
Number of Genes	228
Number of tRNAs	38
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Braelyn_1 Braelyn_2 Braelyn_3 Braelyn_4 Braelyn_5 Braelyn_6 Braelyn_7 (Lsr2-like DNA bridging protein) Braelyn_8 Braelyn_9 Braelyn_10 (ParB-like nuclease domain protein) Braelyn_11 Braelyn_12 Braelyn_13 Braelyn_14 Braelyn_15 Braelyn_16 Braelyn_17 Braelyn_18 Braelyn_19 Braelyn_20 Braelyn_21 Braelyn_22 (HNH endonuclease) Braelyn_23 Braelyn_24 (ThyX-like thymidylate synthase) Braelyn_25 (LAGLIDADG endonuclease) Braelyn_26 Braelyn_27 (hydrolase) Braelyn_28 Braelyn_29 Braelyn_30 Braelyn_31 Braelyn_32 Braelyn_33 Braelyn_34 Braelyn_35 — tRNA Braelyn_36 (HNH endonuclease) Braelyn_37 (endolysin) Braelyn_38 Braelyn_39 Braelyn_40 (glycosyltransferase) Braelyn_41 (ribonucleotide reductase) Braelyn_42 (LysM-like peptidoglycan binding protein) Braelyn_43 (HNH endonuclease) Braelyn_44 Braelyn_45 Braelyn_46 (portal protein) Braelyn_47 Braelyn_48 (capsid maturation protease) Braelyn_49 (major capsid protein) Braelyn_50 Braelyn_51 Braelyn_52 Braelyn_53 Braelyn_54 Braelyn_55 (major tail protein) Braelyn_56 (tail assembly chaperone) Braelyn_57 (tail assembly chaperone) Braelyn_58 (tape measure protein) Braelyn_59 Braelyn_60 (minor tail protein) Braelyn_61 Braelyn_62 (minor tail protein) Braelyn_63 (minor tail protein) Braelyn_64 Braelyn_65 Braelyn_66 Braelyn_67 (HNH endonuclease) Braelyn_68 (metallophosphoesterase) Braelyn_69 Braelyn_70 Braelyn_71 (helix-turn-helix DNA-binding protein) Braelyn_72 Braelyn_73 Braelyn_74 Braelyn_75 (DNA helicase) Braelyn_76 Braelyn_77 (DNA primase) Braelyn_78 (DNA binding protein) Braelyn_79 (DnaE-like DNA polymerase III alpha) Braelyn_80 Braelyn_81 (MazG-like nucleotide pyrophosphohydrolase) Braelyn_82 Braelyn_83 (RecA-like DNA recombinase) Braelyn_84 Braelyn_85 (Holliday junction resolvase) Braelyn_86 Braelyn_87 Braelyn_88 (Cas4 family exonuclease) Braelyn_89 Braelyn_90 (RuvC-like resolvase) Braelyn_91 (DprA-like ssDNA binding protein) Braelyn_92 Braelyn_93 (methyltransferase) Braelyn_94 (thymidylate kinase) Braelyn_95 Braelyn_96 Braelyn_97 (terminase) Braelyn_98 (HNH endonuclease) Braelyn_99 Braelyn_100 Braelyn_101 — tRNA Braelyn_102 — tRNA Braelyn_103 — tRNA Braelyn_104 Braelyn_105 Braelyn_106 — tRNA Braelyn_107 — tRNA Braelyn_108 (nucleotidyltransferase) Braelyn_109 (FtsK-like DNA translocase) Braelyn_110 Braelyn_111 — tRNA Braelyn_112 Braelyn_113 Braelyn_114 Braelyn_115 — tRNA Braelyn_116 Braelyn_117 Braelyn_118 Braelyn_119 — tRNA Braelyn_120 Braelyn_121 Braelyn_122 — tRNA Braelyn_123 Braelyn_124 Braelyn_125 (Ro-like RNA binding protein) Braelyn_126 Braelyn_127 Braelyn_128 Braelyn_129 Braelyn_130 Braelyn_131 Braelyn_132 Braelyn_133 — tRNA Braelyn_134 Braelyn_135 — tRNA Braelyn_136 Braelyn_137 — tRNA Braelyn_138 — tRNA Braelyn_139 — tRNA Braelyn_140 — tRNA Braelyn_141 — tRNA Braelyn_142 Braelyn_143 Braelyn_144 Braelyn_145 Braelyn_146 — tRNA Braelyn_147 — tRNA Braelyn_148 (peptidase) Braelyn_149 Braelyn_150 — tRNA Braelyn_151 Braelyn_152 Braelyn_153 — tRNA Braelyn_154 Braelyn_155 Braelyn_156 — tRNA Braelyn_157 Braelyn_158 — tRNA Braelyn_159 — tRNA Braelyn_160 — tRNA Braelyn_161 (HNH endonuclease) Braelyn_162 — tRNA Braelyn_163 (HNH endonuclease) Braelyn_164 Braelyn_165 — tRNA Braelyn_166 — tRNA Braelyn_167 Braelyn_168 Braelyn_169 Braelyn_170 — tRNA Braelyn_171 — tRNA Braelyn_172 — tRNA Braelyn_173 Braelyn_174 Braelyn_175 — tRNA Braelyn_176 — tRNA Braelyn_177 — tRNA Braelyn_178 Braelyn_179 (DnaQ-like DNA polymerase III subunit) Braelyn_180 — tmRNA Braelyn_181 (hydrolase) Braelyn_182 Braelyn_183 Braelyn_184 (acetyltransferase) Braelyn_185 Braelyn_186 Braelyn_187 Braelyn_188 Braelyn_189 Braelyn_190 (RNA ligase) Braelyn_191 Braelyn_192 Braelyn_193 Braelyn_194 (polynucleotide kinase) Braelyn_195 Braelyn_196 Braelyn_197 — tRNA Braelyn_198 Braelyn_199 Braelyn_200 — tRNA Braelyn_201 Braelyn_202 Braelyn_203 — tRNA Braelyn_204 Braelyn_205 Braelyn_206 Braelyn_207 Braelyn_208 Braelyn_209 Braelyn_210 Braelyn_211 Braelyn_212 Braelyn_213 (deoxycytidylate deaminase) Braelyn_214 Braelyn_215 (rIIB-like protein) Braelyn_216 (phosphoesterase) Braelyn_217 Braelyn_218 Braelyn_219 (WhiB family transcription factor) Braelyn_220 Braelyn_221 Braelyn_222 Braelyn_223 Braelyn_224 Braelyn_225 Braelyn_226 Braelyn_227 Braelyn_228 — tRNA Braelyn_229 Braelyn_230 Braelyn_231 Braelyn_232 Braelyn_233 Braelyn_234 Braelyn_235 (thioredoxin) Braelyn_236 Braelyn_237 Braelyn_238 Braelyn_239 Braelyn_240 Braelyn_241 Braelyn_242 Braelyn_243 Braelyn_244 Braelyn_245 Braelyn_246 Braelyn_247 Braelyn_248 Braelyn_249 Braelyn_250 Braelyn_251 Braelyn_252 (Lsr2-like DNA bridging protein) Braelyn_253 Braelyn_254 Braelyn_255 (ParB-like nuclease domain protein) Braelyn_256 Braelyn_257 Braelyn_258 Braelyn_259 Braelyn_260 Braelyn_261 Braelyn_262 Braelyn_263 Braelyn_264 Braelyn_265 Braelyn_266 Braelyn_267 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN096371
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	81
Pitt Freezer Box Grid#	F12
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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