Streptomyces phage Enygma
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| Detailed Information for Phage Enygma | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Former names | ThisIsPatrick |
| Found By | Timothy Agnes |
| Year Found | 2020 |
| Location Found | Catonsville, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.243826 N, 76.722214 W Map |
| Discovery Notes | Collected in Patapsco State Park in the Avalon area from very moist soil, root filled, surround by organic material, ~3" deep. Collected on 9-02-20. |
| Naming Notes | Voted on by the class |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 830 |
| Genome length (bp) | 133969 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 11545 bp |
| GC Content | 48.5% |
| Sequencing Notes | In about 60% of the sequencing reads for this phage, a 4413 bp deletion (positions 89642 to 94054, inclusive) was observed. Though the population with the deletion is technically the "majority", we've reported the longer sequence as the final genome sequence, otherwise that deleted bit would be lost. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Monmouth College |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 248 |
| Number of tRNAs | 43 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP358745 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.44e12 pfu/mL |
| Pitt Freezer Box# | 117 |
| Pitt Freezer Box Grid# | A3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
