The Actinobacteriophage Database

Arthrobacter phage LittleTokyo

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Detailed Information for Phage LittleTokyo
Discovery Information
Isolation Host	Arthrobacter globiformis B-2979
Found By	Oscar Lim
Year Found	2019
Location Found	Los Angeles, CA United States
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	34.05 N, 118.24 W Map
Discovery Notes	Arthrobacter phage LittleTokyo was isolated from soil by direct plating at 30°C. The sample was collected by Oscar Lim from the Little Tokyo district of Los Angeles, CA near a corner of a shopping district outdoors. LIttleTokyo was isolated, characterized, and annotated by Oscar Lim, Shirley Zhang, Emily Pham, Daniel Peters, Tim Chiang, Kyra Forsyth, Laura Knittig, Shile Zhang and Emily Sardina (UCLA). Instructor: Dr. Amanda Freise Teaching Assistants: Malcolm Phung, Andrew Kapinos Undergraduate Assistants: Andrew Ly & Karina Haleblian
Naming Notes	It was named LittleTokyo because it was discovered in the Little Tokyo district of Los Angeles.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 21, 2019
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	182
Genome length (bp)	38969
Character of genome ends	3' Sticky Overhang
Overhang Length	12 bases
Overhang Sequence	GAGTTGCCGGCA
GC Content	66.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	AS
Subcluster	AS2
Cluster Life Cycle	Temperate
Lysogeny Notes	tyrosine integrase identified.
Other Cluster Members	Click to View Abidatro (AS1) Basilisk (AS1) Brynnie (AS1) Chickaboom (AS1) Eesa (AS1) Galaxy (AS1) Gravel (AS1) Jamun (AS1) KendraB23 (AS1) Orcanus (AS1) Pelletreau (AS1) Ruchi (AS1) TaylorSipht (AS1) Toad24 (AS1) Vulpecula (AS1) Westrich (AS1) WileyE (AS1) Amelia (AS2) Antrice (AS2) Bedetta (AS2) Bibble12 (AS2) Colusalem (AS2) Coral (AS2) Cote (AS2) Cygnet (AS2) Daob (AS2) HannahPhantana (AS2) Jerole (AS2) Kepler (AS2) Kuleana (AS2) LittleTokyo (AS2) Lunar (AS2) Melons (AS2) OtsoOtso (AS2) Polka (AS2) Zhuangyuan (AS2) AmiCi24 (AS3) Andrew (AS3) Atlantica (AS3) Babushka (AS3) Camara (AS3) DanHam62 (AS3) Fingolfin (AS3) Glotell (AS3) HamCheese (AS3) Juno112 (AS3) KHumphrey (AS3) Leona (AS3) PhluffyCoco (AS3) Rattail (AS3) RedFox (AS3) Renna12 (AS3) StuartMinion (AS3)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Plaque Notes	Clear and turbid plaques. Average diameter: 1.5 mm
Morphotype	Siphoviridae
Number of Genes	73
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View LittleTokyo_1 (terminase large subunit, ATPase domain) LittleTokyo_2 LittleTokyo_3 (terminase) LittleTokyo_4 (RNA binding protein) LittleTokyo_5 (portal protein) LittleTokyo_6 (capsid maturation protease) LittleTokyo_7 (major capsid protein) LittleTokyo_8 LittleTokyo_9 (head-to-tail adaptor) LittleTokyo_10 (head-to-tail stopper) LittleTokyo_11 LittleTokyo_12 (tail terminator) LittleTokyo_13 (major tail protein) LittleTokyo_14 (tail assembly chaperone) LittleTokyo_15 (tail assembly chaperone) LittleTokyo_16 (tape measure protein) LittleTokyo_17 (minor tail protein) LittleTokyo_18 (minor tail protein) LittleTokyo_19 (minor tail protein) LittleTokyo_20 (minor tail protein) LittleTokyo_21 LittleTokyo_22 LittleTokyo_23 (endolysin) LittleTokyo_24 LittleTokyo_25 LittleTokyo_26 (helix-turn-helix DNA-binding domain protein) LittleTokyo_27 LittleTokyo_28 LittleTokyo_29 LittleTokyo_30 LittleTokyo_31 LittleTokyo_32 (HNH endonuclease) LittleTokyo_33 (helix-turn-helix DNA-binding domain protein) LittleTokyo_34 LittleTokyo_35 LittleTokyo_36 (tyrosine integrase) LittleTokyo_37 (immunity repressor) LittleTokyo_38 (helix-turn-helix DNA binding domain protein) LittleTokyo_39 (helix-turn-helix DNA-binding domain protein) LittleTokyo_40 (RecE-like exonuclease) LittleTokyo_41 (RecT-like DNA pairing protein) LittleTokyo_42 (helix-turn-helix DNA-binding domain protein) LittleTokyo_43 (RusA-like resolvase) LittleTokyo_44 (helix-turn-helix DNA binding domain protein) LittleTokyo_45 (DNA methylase) LittleTokyo_46 LittleTokyo_47 LittleTokyo_48 LittleTokyo_49 LittleTokyo_50 LittleTokyo_51 (deoxycytidylate deaminase) LittleTokyo_52 (ssDNA-binding protein) LittleTokyo_53 (NucT-like nuclease) LittleTokyo_54 LittleTokyo_55 LittleTokyo_56 LittleTokyo_57 LittleTokyo_58 LittleTokyo_59 LittleTokyo_60 LittleTokyo_61 LittleTokyo_62 LittleTokyo_63 LittleTokyo_64 LittleTokyo_65 LittleTokyo_66 LittleTokyo_67 LittleTokyo_68 LittleTokyo_69 LittleTokyo_70 LittleTokyo_71 (helix-turn-helix DNA-binding domain protein) LittleTokyo_72 (PAPS reductase-like domain protein) LittleTokyo_73 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN703409
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	116
Pitt Freezer Box Grid#	C9
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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