Streptomyces phage Mildred21
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Detailed Information for Phage Mildred21
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByJordyn Chapman and Christy Chang
Year Found2016
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature27°C
GPS Coordinates38.646539 N, 90.303091 W Map
Naming NotesWe found this phage behind the Mildred Lane Kemper Art Museum.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 21, 2017
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage195
Genome length (bp)131976
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length10818 bp
GC Content49.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall plaques with clear centers and cloudy edges
Number of Genes234
Number of tRNAs48
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Mildred21_1

Mildred21_2

Mildred21_3

Mildred21_4

Mildred21_5

Mildred21_6

Mildred21_7
(Lsr2-like DNA bridging protein)

Mildred21_8

Mildred21_9

Mildred21_10
(ParB-like nuclease domain protein)

Mildred21_11
(ParB-like nuclease domain protein)

Mildred21_12

Mildred21_13

Mildred21_14

Mildred21_15

Mildred21_16

Mildred21_17

Mildred21_18

Mildred21_19

Mildred21_20

Mildred21_21

Mildred21_22
(HNH endonuclease)

Mildred21_23

Mildred21_24

Mildred21_25
(ThyX-like thymidylate synthase)

Mildred21_26

Mildred21_27

Mildred21_28

Mildred21_29

Mildred21_30

Mildred21_31

Mildred21_32

Mildred21_33

Mildred21_34

Mildred21_35

Mildred21_36 — tRNA
(tRNA-Thr(cgt))

Mildred21_37
(HNH endonuclease)

Mildred21_38
(endolysin)

Mildred21_39

Mildred21_40
(glycosyltransferase)

Mildred21_41
(ribonucleotide reductase)

Mildred21_42
(LysM-like peptidoglycan-binding protein)

Mildred21_43

Mildred21_44

Mildred21_45
(portal protein)

Mildred21_46

Mildred21_47
(capsid maturation protease)

Mildred21_48
(major capsid protein)

Mildred21_49

Mildred21_50

Mildred21_51

Mildred21_52

Mildred21_53

Mildred21_54

Mildred21_55

Mildred21_56

Mildred21_57
(tape measure protein)

Mildred21_58

Mildred21_59
(minor tail protein)

Mildred21_60

Mildred21_61
(minor tail protein)

Mildred21_62
(minor tail protein)

Mildred21_63

Mildred21_64

Mildred21_65

Mildred21_66
(HNH endonuclease)

Mildred21_67
(metallophosphoesterase)

Mildred21_68

Mildred21_69

Mildred21_70

Mildred21_71

Mildred21_72

Mildred21_73

Mildred21_74
(DNA helicase)

Mildred21_75

Mildred21_76
(DNA primase)

Mildred21_77

Mildred21_78
(DnaE-like DNA polymerase III alpha)

Mildred21_79

Mildred21_80
(MazG-like nucleotide pyrophosphohydrolase)

Mildred21_81
(RecA-like DNA recombinase)

Mildred21_82

Mildred21_83
(holliday junction resolvase)

Mildred21_84

Mildred21_85

Mildred21_86
(Cas4 family exonuclease)

Mildred21_87

Mildred21_88
(RuvC-like resolvase)

Mildred21_89
(DprA-like ssDNA binding protein)

Mildred21_90
(helix-turn-helix DNA-binding protein)

Mildred21_91
(methyltransferase)

Mildred21_92
(thymidylate kinase)

Mildred21_93

Mildred21_94

Mildred21_95
(terminase)

Mildred21_96
(HNH endonuclease)

Mildred21_97

Mildred21_98

Mildred21_99 — tRNA
(tRNA-Gly(tcc))

Mildred21_100 — tRNA
(tRNA-Gln(ctg))

Mildred21_101 — tRNA
(tRNA-Trp(cca))

Mildred21_102

Mildred21_103 — tRNA
(tRNA-Pro(tgg))

Mildred21_104

Mildred21_105 — tRNA
(tRNA-Pro(tgg))

Mildred21_106
(nucleotidyltransferase)

Mildred21_107
(FtsK-like DNA translocase)

Mildred21_108

Mildred21_109 — tRNA
(tRNA-Ile(tat))

Mildred21_110

Mildred21_111

Mildred21_112

Mildred21_113 — tRNA
(tRNA-Ser(gga))

Mildred21_114

Mildred21_116 — tRNA
(tRNA-Ser(tga))

Mildred21_117
(HNH endonuclease)

Mildred21_118

Mildred21_119

Mildred21_120

Mildred21_121

Mildred21_122 — tRNA
(tRNA-Asp(gtc))

Mildred21_123

Mildred21_124

Mildred21_125

Mildred21_126

Mildred21_127 — tRNA
(tRNA-Asp(gtc))

Mildred21_128 — tRNA
(tRNA-Ser(tga))

Mildred21_129

Mildred21_130

Mildred21_131
(Ro-like RNA binding protein)

Mildred21_132

Mildred21_133

Mildred21_134

Mildred21_135

Mildred21_136

Mildred21_137

Mildred21_138 — tRNA
(tRNA-Glu(ctc))

Mildred21_139 — tRNA
(tRNA-Glu(ttc))

Mildred21_140 — tRNA
(tRNA-Val(tac))

Mildred21_141 — tRNA
(tRNA-Leu(tag))

Mildred21_142
(HNH endonuclease)

Mildred21_143 — tRNA
(tRNA-Leu(tag))

Mildred21_144 — tRNA
(tRNA-Tyr(gta))

Mildred21_145 — tRNA
(tRNA-Phe(gaa))

Mildred21_146 — tRNA
(tRNA-Gly(gcc))

Mildred21_147 — tRNA

Mildred21_148

Mildred21_149

Mildred21_150

Mildred21_151 — tRNA
(tRNA-Ala(tgc))

Mildred21_152 — tRNA
(tRNA-Lys(ctt))

Mildred21_153

Mildred21_154

Mildred21_155

Mildred21_156 — tRNA
(tRNA-Asn(gtt))

Mildred21_157

Mildred21_159 — tRNA
(tRNA-Asn(gtt))

Mildred21_160

Mildred21_161

Mildred21_162 — tRNA
(tRNA-Arg(cct))

Mildred21_163

Mildred21_164 — tRNA
(tRNA-Ile(gat))

Mildred21_165 — tRNA
(tRNA-Ala(ggc))

Mildred21_166 — tRNA
(tRNA-Lys(ctt))

Mildred21_167 — tRNA
(tRNA-Lys(ttt))

Mildred21_168
(HNH endonuclease)

Mildred21_169 — tRNA
(tRNA-Phe(gaa))

Mildred21_170

Mildred21_171 — tRNA
(tRNA-Met(cat))

Mildred21_172 — tRNA
(tRNA-Met(cat))

Mildred21_173

Mildred21_174

Mildred21_175 — tRNA
(tRNA-Tyr(gta))

Mildred21_176 — tRNA
(tRNA-Tyr(gta))

Mildred21_177 — tRNA
(tRNA-His(gtg))

Mildred21_178 — tRNA
(tRNA-His(gtg))

Mildred21_179 — tRNA
(tRNA-Phe(gaa))

Mildred21_180

Mildred21_181

Mildred21_182 — tRNA
(tRNA-Cys(gca))

Mildred21_183 — tRNA
(tRNA-Thr(tgt))

Mildred21_184 — tRNA
(tRNA-Thr(ggt))

Mildred21_185

Mildred21_186

Mildred21_187
(DnaQ-like DNA polymerase III subunit)

Mildred21_188

Mildred21_189

Mildred21_190 — tmRNA

Mildred21_191

Mildred21_192

Mildred21_193

Mildred21_194

Mildred21_195

Mildred21_196

Mildred21_197 — tRNA

Mildred21_198

Mildred21_199 — tRNA

Mildred21_200

Mildred21_201

Mildred21_202

Mildred21_203
(RNA ligase)

Mildred21_204

Mildred21_205

Mildred21_206
(polynucleotide kinase)

Mildred21_207

Mildred21_208

Mildred21_209 — tRNA
(tRNA-Leu(gag))

Mildred21_210

Mildred21_211

Mildred21_212 — tRNA
(tRNA-Val(gac))

Mildred21_213

Mildred21_214 — tRNA
(tRNA-Leu(caa))

Mildred21_215

Mildred21_216 — tRNA
(tRNA-Ser(gct))

Mildred21_217

Mildred21_218

Mildred21_219

Mildred21_220

Mildred21_221

Mildred21_222

Mildred21_223

Mildred21_224

Mildred21_225

Mildred21_226
(deoxycytidylate deaminase)

Mildred21_227

Mildred21_228
(rIIB-like protein)

Mildred21_229
(phosphoesterase)

Mildred21_230

Mildred21_231

Mildred21_232
(HNH endonuclease)

Mildred21_233
(WhiB family transcription factor)

Mildred21_234

Mildred21_235

Mildred21_236

Mildred21_237

Mildred21_238

Mildred21_239

Mildred21_240

Mildred21_241

Mildred21_242

Mildred21_243

Mildred21_244 — tRNA
(tRNA-Gln(ttg))

Mildred21_245

Mildred21_246

Mildred21_247

Mildred21_248

Mildred21_249

Mildred21_250

Mildred21_251

Mildred21_252

Mildred21_253
(thioredoxin)

Mildred21_254

Mildred21_255

Mildred21_256

Mildred21_257

Mildred21_258

Mildred21_259

Mildred21_260

Mildred21_261

Mildred21_262

Mildred21_263

Mildred21_264

Mildred21_265

Mildred21_266

Mildred21_267

Mildred21_268

Mildred21_269

Mildred21_270
(Lsr2-like DNA bridging protein)

Mildred21_271

Mildred21_272

Mildred21_273
(ParB-like nuclease domain protein)

Mildred21_274
(ParB-like nuclease domain protein)

Mildred21_275

Mildred21_276

Mildred21_277

Mildred21_278

Mildred21_279

Mildred21_280

Mildred21_281

Mildred21_282

Mildred21_283

Mildred21_284

Mildred21_285
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF155946
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 9.5 x 10^9 pfu/mL
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload