| Detailed Information for Phage PhillyPhilly |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Jack Kisielius |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 24°C |
| GPS Coordinates | 39.945098 N, 75.207592 W Map |
| Discovery Notes | Little to no foot traffic from where the sample was collected. Exposed to sunlight; on top of a ridge; and near train tracks. |
| Naming Notes | We named our phage PhillyPhilly in order to forever commemorate the Philadelphia Eagles winning Super Bowl 52. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 8, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1492 |
| Genome length (bp) | 62869 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3380 bp |
| GC Content | 62.7% |
| Sequencing Notes | This phage was sequenced as part of a mixed DOGEMS sample and later identified by PCR. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | ED |
| Subcluster | ED1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | Multiple plaque sizes, one size was 1 mm and the other plaque size was around 2.5 mm. Clear plaques were formed. |
| Morphotype | Siphoviridae |
| Number of Genes | 118 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK937596 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.8 x 10^9 pfu/mL |
| Pitt Freezer Box# |
84 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
