Detailed Information for Phage Sadboi |
Discovery Information |
Isolation Host | Gordonia rubripertincta NRRL B-16540 |
Found By | Michael Lauer |
Year Found | 2018 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Phage Hunters Integrating Research and Education |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 40.447276 N, 79.953995 W Map |
Discovery Notes | found in a bed of flowers at the University of Pittsburgh outside of Crawford Hall. Each sample in the series was taken from different holes in the flowerbed. |
Naming Notes | A catch phrase that I coined when you're having a depressing day; you are sadboi for the day. It is also used to reference people born in 1998; they are sadboi_98. I hope that this phage has 98 genes so that there will be a sadboi_98 gene. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jul 24, 2018 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 711 |
Genome length (bp) | 67485 |
Character of genome ends | Circularly Permuted |
GC Content | 58.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | DV |
Subcluster | -- |
Cluster Life Cycle | Unknown |
Lysogeny Notes | Lysate diluted and spotted, no mesas after 5 days. The center of the lytic clearings were picked and steaked on a PYCa plate in a patch test, and no lysogenic cells grew. |
Other Cluster Members |
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Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | lytic, small, pinhole-like |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MN234232 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.0X10^11 pfu/mL |
Pitt Freezer Box# |
96 |
Pitt Freezer Box Grid# |
E8 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |