| Detailed Information for Phage Sadboi | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Michael Lauer |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.447276 N, 79.953995 W Map |
| Discovery Notes | found in a bed of flowers at the University of Pittsburgh outside of Crawford Hall. Each sample in the series was taken from different holes in the flowerbed. |
| Naming Notes | A catch phrase that I coined when you're having a depressing day; you are sadboi for the day. It is also used to reference people born in 1998; they are sadboi_98. I hope that this phage has 98 genes so that there will be a sadboi_98 gene. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 24, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 711 |
| Genome length (bp) | 67485 |
| Character of genome ends | Circularly Permuted |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DV |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Lysate diluted and spotted, no mesas after 5 days. The center of the lytic clearings were picked and steaked on a PYCa plate in a patch test, and no lysogenic cells grew. |
| Other Cluster Members |
Click to ViewAlephilan Alyssamiracle Apeppi Astralis Avian BirthdayBoy DaviePasture DoobyDoo DumpTruck Erutan Fulcrum Genamy16 Gibbin GOATification GretelLyn Jalebi Jamemuya19 Lambo LavAbarElk Lila22 LuckyLeo Mima20 MoiGyank NorManre NovaSharks OtterstedtS21 ParvusTarda Patos Ranch RazorC Rumi Sadboi Sampudon Stormer Tillicus Wojtek Xenia2 Yikes Zany |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | lytic, small, pinhole-like |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSadboi_1 Sadboi_2 Sadboi_3 Sadboi_4 Sadboi_5 Sadboi_6 Sadboi_7 Sadboi_8 Sadboi_9 Sadboi_10 Sadboi_11 Sadboi_12 Sadboi_13 Sadboi_14 Sadboi_15 Sadboi_16 Sadboi_17 Sadboi_18 Sadboi_19 Sadboi_20 Sadboi_21 Sadboi_22 Sadboi_23 Sadboi_24 Sadboi_25 Sadboi_26 Sadboi_27 Sadboi_28 Sadboi_29 Sadboi_30 Sadboi_31 Sadboi_32 Sadboi_33 Sadboi_34 Sadboi_35 Sadboi_36 Sadboi_37 Sadboi_38 Sadboi_39 Sadboi_40 Sadboi_41 Sadboi_42 Sadboi_43 Sadboi_44 Sadboi_45 Sadboi_46 Sadboi_47 Sadboi_48 Sadboi_49 Sadboi_50 Sadboi_51 Sadboi_52 Sadboi_53 Sadboi_54 Sadboi_55 Sadboi_56 Sadboi_57 Sadboi_58 Sadboi_59 Sadboi_60 Sadboi_61 Sadboi_62 Sadboi_63 Sadboi_64 Sadboi_65 Sadboi_66 Sadboi_67 Sadboi_68 Sadboi_69 Sadboi_70 Sadboi_71 Sadboi_72 Sadboi_73 Sadboi_74 Sadboi_75 Sadboi_76 Sadboi_77 Sadboi_78 Sadboi_79 Sadboi_80 Sadboi_81 Sadboi_82 Sadboi_83 Sadboi_84 Sadboi_85 Sadboi_86 Sadboi_87 Sadboi_88 Sadboi_89 Sadboi_90 Sadboi_91 Sadboi_92 Sadboi_93 Sadboi_94 Sadboi_95 Sadboi_96 Sadboi_97 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234232 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.0X10^11 pfu/mL |
| Pitt Freezer Box# | 96 |
| Pitt Freezer Box Grid# | E8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
