| Detailed Information for Phage Seume | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Bre Krohn and Bri Phernetton |
| Year Found | 2013 |
| Location Found | Somerset, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 45.166643 N, 92.717961 W Map |
| Discovery Notes | Soil sample was taken from a tomato garden on 9-8-13 at 9:43 AM. The temperature at the time of the collection was humid and partly sunny.The soil was taken from the ground 50 mm deep and it was loose and mostly dry. |
| Naming Notes | The phage is named after Bri Phernetton's high school science teacher, who is one of the most influential and admirable people in her life. He inspired her to pursue a science major and will always be a role model. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 20, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 12826 |
| Genome length (bp) | 15319 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 60.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewArby Arielagos Azathoth Blair CGermain Chestnut Copper Courtney3 Decurro Dewayne Elkhorn Guntur Hunnie Inspire2 Jessica Kels KylieMac Laila Link Lore LouisXIV Maggie Mariposa Massimo Moloch Muttlie Prospero Ronnie Sandman Saphira SerialPhiller Seume Sourignavong StewieGriff Stratus Swenson Taj14 TinoCrisci Toulouse TymAbreu Yank |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | the plaques are about 1 cm in diameter including a faint halo that surrounds each plaque. Similiar to Klycomp, Katherine, and Poppy |
| Morphotype | Siphoviridae |
| Number of Genes | 26 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSeume_1 Seume_2 Seume_3 Seume_4 Seume_5 Seume_6 Seume_7 Seume_8 Seume_9 Seume_10 Seume_11 Seume_12 Seume_13 Seume_14 Seume_15 Seume_16 Seume_17 Seume_18 Seume_19 Seume_20 Seume_21 Seume_22 Seume_23 Seume_24 Seume_25 Seume_26 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140426 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.5x10^10 |
| Date of SEA Lysate Titering | Oct 17, 2013 |
| Pitt Freezer Box# | 147 |
| Pitt Freezer Box Grid# | G11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
