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Streptomyces phage Kromp
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Detailed Information for Phage Kromp
Discovery Information
Isolation HostStreptomyces lividans JI 1326
Former namesJ9DmycrowL
Found ByMuhammad Mousa and Benjamin Kelsky
Year Found2017
Location FoundSt. Louis, MO United States
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates38.6409 N, 90.2992 W Map
Discovery NotesSample collected in Forest Park, from an area with dense moss and rotting grass. Sample was moist, full of organic matter, and possessed a strong scent. Collected at 28 degrees Celsius at a depth of 8 centimeters.
Naming NotesThis phage was named for Krombopulos Michael, who deserves to be honored.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 12, 2018
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage300
Genome length (bp)58268
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCGGGGGA
GC Content71.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Lysogeny NotesSee plaque morphology notes
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques range from <1 to 5 mm in diameter. Often a small number of surviving cells is present within the plaque, these cells exclusively express the gene for red pigment. It is hypothesized (but still unverified) that these cells are lysogenically infected, and that the lysogenic Kromp infection is responsible for this expression.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Kromp_1
(terminase small subunit)

Kromp_2
(terminase large subunit)

Kromp_3
(portal protein)

Kromp_4
(capsid maturation protease)

Kromp_5
(scaffolding protein)

Kromp_6
(protease)

Kromp_7
(major capsid protein)

Kromp_8

Kromp_9
(head-to-tail connector complex protein)

Kromp_10
(head-to-tail connector complex protein)

Kromp_11

Kromp_12

Kromp_13
(head-to-tail connector complex protein)

Kromp_14
(major tail protein)

Kromp_15
(tail assembly chaperone)

Kromp_16
(tail assembly chaperone)

Kromp_17
(tape measure protein)

Kromp_18
(minor tail protein)

Kromp_19
(minor tail protein)

Kromp_20

Kromp_21
(minor tail protein)

Kromp_22

Kromp_23

Kromp_24

Kromp_25
(LysM-like endolysin)

Kromp_26
(holin)

Kromp_27

Kromp_28
(minor tail protein)

Kromp_29

Kromp_30

Kromp_31

Kromp_32

Kromp_33

Kromp_34

Kromp_35
(helix-turn-helix DNA-binding domain protein)

Kromp_36
(integrase)

Kromp_37
(helix-turn-helix DNA-binding domain protein)

Kromp_38
(immunity repressor)

Kromp_39

Kromp_40

Kromp_41

Kromp_42

Kromp_43

Kromp_44

Kromp_45

Kromp_46

Kromp_47
(helix-turn-helix DNA-binding domain protein)

Kromp_48

Kromp_49

Kromp_50
(helix-turn-helix DNA-binding domain protein)

Kromp_51

Kromp_52
(WhiB family transcription factor)

Kromp_53

Kromp_54

Kromp_55

Kromp_56

Kromp_57

Kromp_58

Kromp_59

Kromp_60

Kromp_61

Kromp_62

Kromp_63
(helix-turn-helix DNA-binding domain protein)

Kromp_64

Kromp_65

Kromp_66

Kromp_67

Kromp_68

Kromp_69

Kromp_70

Kromp_71

Kromp_72

Kromp_73

Kromp_74
(RNA polymerase sigma factor)

Kromp_75

Kromp_76

Kromp_77

Kromp_78

Kromp_79
(glycosyltransferase)

Kromp_80

Kromp_81

Kromp_82

Kromp_83

Kromp_84

Kromp_85

Kromp_86

Kromp_87

Kromp_88

Kromp_89
(MerR-like HTH DNA binding protein)

Kromp_90

Kromp_91

Kromp_92

Kromp_93

Kromp_94

Kromp_95
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH744420
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.01*10^11
Pitt Freezer Box# 49
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload