| Detailed Information for Phage Samy |
| Discovery Information |
| Isolation Host | Streptomyces ambofaciens ATCC 23877 |
| Found By | Stéphanie Bury-Moné |
| Year Found | 2023 |
| Location Found | Péronne, France |
| Finding Institution | University Paris-Saclay |
| Program | Unavailable |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 49.93333 N, 2.93333 E Map |
| Discovery Notes | Samy is a temperate phage present in the genome of Streptomyces ambofaciens ATCC 23877. Samy particles were isolated from cultures of this strain grown for 4 days at 30°C in BM medium (1 g/l yeast extract, 1 g/l meat/beef extract, 2 g/l bactotryptone, pH 7.3). Samy is produced produced simultaneously with the activation of other mobile genetic elements and phage-related genes such as those encoding phage tail-like nanostructures. |
| Naming Notes | The name was chosen because of the name of the host strain Streptomyces ambofaciens (SAM) ATCC 23877, which already contains other mobile genetic elements, plasmids called pSAM1 and pSAM2. The phage produced was therefore named Samy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2024 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2558 |
| Genome length (bp) | 61068 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGTTAAGGTGC |
| GC Content | 66.5% |
| Sequencing Notes | There is one base in the PhagesDB genome of this sequence that differs from the sequence currently in GenBank. At position 55286, we have a G where the GenBank file has a T. This is because the sequencing reads showed a polymorphism at that position, and we called a G (~59% of reads) whereas the previous assembly called a T (~41% of reads). Neither is "right" or "wrong", as the population that was sequenced was truly mixed at this position. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University Paris-Saclay |
| Annotation Status | In GenBank |
| Plaque Notes | Very small plaques (< 1 mm).
Lab host: Streptomyces lividans TK24 |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 4 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR263580 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
