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Mycobacterium phage Kumao
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Detailed Information for Phage Kumao
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLisa Sako-Glover
Year Found2015
Location FoundEaston, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-1°C
GPS Coordinates40.0 N, 75.0 W Map
Discovery NotesIsolated from wet soil at a depth of 1/2 inch, at the edge of Bushkill Creek in Easton, PA (Forks township). The location of sample collection was under the bridge (north side) of Bushkill Creek Drive at Tatamy Road. The environmental sample was collected on on the morning of January 20, 2015. Despite the winter weather, Bushkill Creek was no frozen and actively flowing.
Naming NotesNamed after my father, Kumao Sako, who was a Cancer research physician. He is my inspiration.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 29, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage501
Genome length (bp)70373
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCCTCGCGAA
GC Content62.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Other Cluster Members
Plaque NotesPlaques were 1mm in size, clear, with an off center halo that was apparent at 48hours.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload