Mycobacterium phage Kumao
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Detailed Information for Phage Kumao
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLisa Sako-Glover
Year Found2015
Location FoundEaston, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-1°C
GPS Coordinates40.0 N, 75.0 W Map
Discovery NotesIsolated from wet soil at a depth of 1/2 inch, at the edge of Bushkill Creek in Easton, PA (Forks township). The location of sample collection was under the bridge (north side) of Bushkill Creek Drive at Tatamy Road. The environmental sample was collected on on the morning of January 20, 2015. Despite the winter weather, Bushkill Creek was no frozen and actively flowing.
Naming NotesNamed after my father, Kumao Sako, who was a Cancer research physician. He is my inspiration.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 29, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage501
Genome length (bp)70373
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCCTCGCGAA
GC Content62.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Other Cluster Members
Plaque NotesPlaques were 1mm in size, clear, with an off center halo that was apparent at 48hours.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes115
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Kumao_1

Kumao_2

Kumao_3

Kumao_4
(DNA binding protein)

Kumao_5

Kumao_6

Kumao_7
(thymidylate synthase)

Kumao_8

Kumao_9

Kumao_10

Kumao_11

Kumao_12
(terminase small subunit)

Kumao_13
(terminase large subunit)

Kumao_14
(portal protein)

Kumao_15
(capsid maturation protease)

Kumao_16
(scaffolding protein)

Kumao_17
(major capsid protein)

Kumao_18
(head-to-tail connector protein)

Kumao_19
(head-to-tail connector protein)

Kumao_20
(head-to-tail connector protein)

Kumao_21
(head-to-tail connector protein)

Kumao_22
(major tail protein)

Kumao_23

Kumao_24
(tail assembly chaperone)

Kumao_25
(tail assembly chaperone)

Kumao_26
(tape measure protein)

Kumao_27
(minor tail protein)

Kumao_28
(minor tail protein)

Kumao_29
(minor tail protein)

Kumao_30
(minor tail protein)

Kumao_31
(D-Ala-D-Ala carboxypeptidase)

Kumao_32
(minor tail protein)

Kumao_33

Kumao_34
(membrane protein)

Kumao_35
(minor tail protein)

Kumao_36

Kumao_37
(lysin A)

Kumao_38
(holin)

Kumao_39
(lysin B)

Kumao_40
(glutaredoxin)

Kumao_41

Kumao_42

Kumao_43

Kumao_44

Kumao_45

Kumao_46
(membrane protein)

Kumao_47
(ERF family ssDNA binding protein)

Kumao_48

Kumao_49
(protease)

Kumao_50

Kumao_51

Kumao_52

Kumao_53

Kumao_54

Kumao_55

Kumao_56

Kumao_57

Kumao_58
(HNH endonuclease)

Kumao_59

Kumao_60

Kumao_61

Kumao_62
(HNH endonuclease)

Kumao_63
(ssDNA binding protein)

Kumao_64

Kumao_65
(DnaB-like dsDNA helicase)

Kumao_66
(WhiB family transcription factor)

Kumao_67
(RusA-like resolvase)

Kumao_68
(WhiB family transcription factor)

Kumao_69

Kumao_70
(5' nucleotidase)

Kumao_71

Kumao_72

Kumao_73

Kumao_74

Kumao_75
(DNA polymerase III subunit)

Kumao_76

Kumao_77
(HNH endonuclease)

Kumao_78

Kumao_79
(HNH endonuclease)

Kumao_81
(DNA binding protein)

Kumao_82

Kumao_83

Kumao_84
(dprA-like single stranded DNA binding protein)

Kumao_85

Kumao_86

Kumao_87
(HNH endonuclease)

Kumao_88
(DNA recombinase)

Kumao_89

Kumao_90

Kumao_91
(nucleotide pyrophosphohydrolase)

Kumao_92

Kumao_93

Kumao_94

Kumao_95
(phosphoesterase)

Kumao_96
(integrase)

Kumao_97
(membrane protein)

Kumao_98

Kumao_99

Kumao_100

Kumao_101

Kumao_102

Kumao_103

Kumao_104

Kumao_105

Kumao_106

Kumao_107

Kumao_108

Kumao_109

Kumao_110

Kumao_111

Kumao_112

Kumao_113

Kumao_114

Kumao_115
(N-glycosylase)

Kumao_116

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG009575
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload