| Detailed Information for Phage ASegato |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Beatriz De Oliveira Segato |
| Year Found | 2022 |
| Location Found | Quincy, MA USA |
| Finding Institution | Eastern Nazarene College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 42.27307 N, 71.01037 W Map |
| Discovery Notes | Soil was dry but the grass was very green, alive and overgrown |
| Naming Notes | My mother's name is Aurea and our family last name is Segato |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 754 |
| Genome length (bp) | 63246 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3797 bp |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | ED |
| Subcluster | ED2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Eastern Nazarene College |
| Annotation Status | In GenBank |
| Plaque Notes | Phages were medium size with a clear center and a cloudy ring around it and clear all around the ring.
Bulls eye phage |
| Morphotype | Siphoviridae |
| Number of Genes | 121 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR521059 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.88x10^8 |
| Pitt Freezer Box# |
152 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
