| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Christian Queen, Mei Zheng |
| Year Found | 2021 |
| Location Found | Glen Burnie, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 39.133021 N, 76.628615 W Map |
| Discovery Notes | This phage was discovered in Glen Burnie, Maryland, USA on 09/14/2021 at a temperature of 21.1º C. The soil where the phage was extracted from was moist and grain-like. |
| Naming Notes | Annihilus is a supervillian from Marvel comics; this was a perfect description of our phage because it annihilated Streptomyces scabiei on our 10^0 from our first round of purification, leaving no trace of the bacteria on the plate. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 6170 |
| Genome length (bp) | 43562 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque diameter was approximately 2.54 mm; there were no turbidity around the plaque, the plaque was clear and circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 59 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON081336 |
| Refseq Number | None yet |
| Published in Paper | Park et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces Bacteriophages Annihilus, TonyStarch, Thiqqums, CricKo, ClubPenguin, RosaAsantewaa, and PherryCruz |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.05 x 10^9 pfu/mL |
| Pitt Freezer Box# |
131 |
| Pitt Freezer Box Grid# |
G6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
