Discovery Information |
Isolation Host | Streptomyces scabiei RL-34 |
Found By | Christian Queen, Mei Zheng |
Year Found | 2021 |
Location Found | Glen Burnie, MD United States |
Finding Institution | University of Maryland, Baltimore County |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 21°C |
GPS Coordinates | 39.133021 N, 76.628615 W Map |
Discovery Notes | This phage was discovered in Glen Burnie, Maryland, USA on 09/14/2021 at a temperature of 21.1º C. The soil where the phage was extracted from was moist and grain-like. |
Naming Notes | Annihilus is a supervillian from Marvel comics; this was a perfect description of our phage because it annihilated Streptomyces scabiei on our 10^0 from our first round of purification, leaving no trace of the bacteria on the plate. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 3, 2022 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 6170 |
Genome length (bp) | 43562 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCCGCCCT |
GC Content | 61.2% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BI |
Subcluster | BI2 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | University of Maryland, Baltimore County |
Annotation Status | In GenBank |
Plaque Notes | Plaque diameter was approximately 2.54 mm; there were no turbidity around the plaque, the plaque was clear and circular. |
Morphotype | Siphoviridae |
Number of Genes | 59 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | ON081336 |
Refseq Number | None yet |
Published in Paper | Park et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces Bacteriophages Annihilus, TonyStarch, Thiqqums, CricKo, ClubPenguin, RosaAsantewaa, and PherryCruz |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
2.05 x 10^9 pfu/mL |
Pitt Freezer Box# |
131 |
Pitt Freezer Box Grid# |
G6 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |