Streptomyces phage HotFries
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Detailed Information for Phage HotFries
Discovery Information
Isolation HostStreptomyces scabiei RL-34
Found ByPatel,Harsh & Patel,Priya
Year Found2017
Location FoundOwings Mills, MD USA
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates39.441938 N, 76.785539 W Map
Discovery NotesTemp 77F; Semi-dry soil found in an empty patch from a garden; around 6 inches deep
Naming NotesHotFries was named after our favorite snack made by Andy Capp's.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 18, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)43699
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCCCT
GC Content60.9%
Sequencing Notesafter position 43651 bp consensus contains string 10 G's, 20% of reads contain 10 or 12 G's
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Lysogeny NotesA mesa was not formed after spotting 10^0-10^-7 dilutions of the lysate.
Other Cluster Members
Annotating InstitutionUniversity of Maryland, Baltimore County
Annotation StatusIn GenBank
Plaque Notes2 mm in diameter, cloudy, round, smooth edges
Number of Genes57
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH155869
Refseq NumberNone yet
Published in PaperBlocker D et al 2018, Genome Announcements: Complete Genome Sequences of Six BI Cluster Streptomyces Bacteriophages, HotFries, Moozy, Rainydai, RavenPuff, Scap1, and SenditCS.
Archiving Info
Archiving status Archived
SEA Lysate Titer 9.9x10^9
Pitt Freezer Box# 49
Pitt Freezer Box Grid# F2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload