Streptomyces phage FidgetOrca
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Detailed Information for Phage FidgetOrca
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByAnne Remorca
Year Found2019
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.208929 N, 97.147668 W Map
Discovery NotesThe soil sample was discovered under a tree near a bench located between the UNT's Business Leadership Building and Crumely Hall. The collection site has light foot traffic and has plenty of leaves, acorns, and organisms (such as squirrels and insects). The weather at the time of discovery was rainy, cloudy, and cold. Though at the time of collection it was not raining, the soil sample was still damp from the night before.
Naming NotesThe name for this phage comes from the people who inspire me. The first half of my phage name comes from what my friends and I jokingly call our friend group, the Fidget Friends, and represents all of the times that my friends have helped and supported me. The second half of my phage name is from the second half of my last name, Remorca, to honor my family who support and encourage me to continue to work hard.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 26, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1672
Genome length (bp)56115
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCCGCCT
GC Content59.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBI
SubclusterBI1
Cluster Life CycleLytic
Lysogeny NotesThe particular sample produced a negative result, however the test does not produce a definite result due to the fact that the chosen sample may not be indicative for all the phage's results. In short, though the particular test produced a negative result, a positive result could be possible with a different test sample.
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesTypically FidgetOrca produced clear, generally round plaques with diameters ranging from 0.1 mm to 0.75 mm. There was some variation with the plaque clarity, with some plaques presenting small bubbles in the center of large plaques, however the rest of the plaque was completely cleared. The plaque shape was also slightly varied with some plaques exhibiting a more oblong shape and many of the plaques exhibiting circular shapes with either a rough (wavy) or smooth outline.
Has been Phamerated?Yes
Gene List

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FidgetOrca_1

FidgetOrca_2
(HNH endonuclease)

FidgetOrca_3

FidgetOrca_4

FidgetOrca_5
(endolysin)

FidgetOrca_6

FidgetOrca_7

FidgetOrca_8
(terminase large subunit)

FidgetOrca_9

FidgetOrca_10

FidgetOrca_11

FidgetOrca_12
(portal protein)

FidgetOrca_13

FidgetOrca_14
(capsid maturation protease)

FidgetOrca_15

FidgetOrca_16
(tail terminator)

FidgetOrca_17
(major tail protein)

FidgetOrca_18

FidgetOrca_19
(major tail protein)

FidgetOrca_20

FidgetOrca_21
(glycoside hydrolase)

FidgetOrca_22

FidgetOrca_23
(tape measure protein)

FidgetOrca_24
(minor tail protein)

FidgetOrca_25
(minor tail protein)

FidgetOrca_26

FidgetOrca_27
(hydrolase)

FidgetOrca_28

FidgetOrca_29
(holin)

FidgetOrca_30

FidgetOrca_31

FidgetOrca_32

FidgetOrca_33

FidgetOrca_34

FidgetOrca_35

FidgetOrca_36

FidgetOrca_37

FidgetOrca_38

FidgetOrca_39

FidgetOrca_40

FidgetOrca_41

FidgetOrca_42

FidgetOrca_43

FidgetOrca_44

FidgetOrca_45

FidgetOrca_46

FidgetOrca_47

FidgetOrca_48

FidgetOrca_49

FidgetOrca_50

FidgetOrca_51

FidgetOrca_52

FidgetOrca_53

FidgetOrca_54

FidgetOrca_55

FidgetOrca_56

FidgetOrca_57

FidgetOrca_58

FidgetOrca_59

FidgetOrca_60
(DNA primase/polymerase)

FidgetOrca_61

FidgetOrca_62

FidgetOrca_63

FidgetOrca_64

FidgetOrca_65

FidgetOrca_66

FidgetOrca_67

FidgetOrca_68

FidgetOrca_69

FidgetOrca_70

FidgetOrca_71

FidgetOrca_72
(DprA-like DNA processing chain A)

FidgetOrca_73

FidgetOrca_74

FidgetOrca_75

FidgetOrca_76

FidgetOrca_77

FidgetOrca_78
(thymidylate kinase)

FidgetOrca_79

FidgetOrca_80

FidgetOrca_81

FidgetOrca_82

FidgetOrca_83
(helicase)

FidgetOrca_84
(HNH endonuclease)

FidgetOrca_85
(hydrolase)

FidgetOrca_86
(DNA helicase)

FidgetOrca_87
(helix-turn-helix DNA binding domain protein)

FidgetOrca_88

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN586056
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^8 pfu/ml
Pitt Freezer Box# 104
Pitt Freezer Box Grid# B2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload