| Detailed Information for Phage FidgetOrca | |
| Discovery Information | |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Anne Remorca |
| Year Found | 2019 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.208929 N, 97.147668 W Map |
| Discovery Notes | The soil sample was discovered under a tree near a bench located between the UNT's Business Leadership Building and Crumely Hall. The collection site has light foot traffic and has plenty of leaves, acorns, and organisms (such as squirrels and insects). The weather at the time of discovery was rainy, cloudy, and cold. Though at the time of collection it was not raining, the soil sample was still damp from the night before. |
| Naming Notes | The name for this phage comes from the people who inspire me. The first half of my phage name comes from what my friends and I jokingly call our friend group, the Fidget Friends, and represents all of the times that my friends have helped and supported me. The second half of my phage name is from the second half of my last name, Remorca, to honor my family who support and encourage me to continue to work hard. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 26, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1672 |
| Genome length (bp) | 56115 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 59.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BI |
| Subcluster | BI1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The particular sample produced a negative result, however the test does not produce a definite result due to the fact that the chosen sample may not be indicative for all the phage's results. In short, though the particular test produced a negative result, a positive result could be possible with a different test sample. |
| Other Cluster Members |
Click to ViewCherryBlossom (BI1) DrGrey (BI1) Esketit (BI1) FidgetOrca (BI1) FrodoSwaggins (BI1) GirlPower (BI1) Hoshi (BI1) IceWarrior (BI1) Indigenous (BI1) Jaylociraptor (BI1) Madamato (BI1) Maya (BI1) Meibysrarus (BI1) Namo (BI1) OlympicHelado (BI1) Popy (BI1) Rima (BI1) Soshi (BI1) Spectropatronm (BI1) TaidaOne (BI1) TieDye (BI1) TonyStarch (BI1) Annihilus (BI2) Bilo (BI2) Doxi13 (BI2) GoblinVoyage (BI2) HotFries (BI2) Moozy (BI2) PherryCruz (BI2) RavenPuff (BI2) RosaAsantewaa (BI2) Scap1 (BI2) SheRa (BI2) LibertyBell (BI3) CricKo (BI4) FromIndy (BI4) Miek (BI4) Rainydai (BI4) Regigigas (BI4) SendItCS (BI4) Thiqqums (BI4) Bing (BI5) Kardashian (BI6) ClubPenguin (BI7) |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Typically FidgetOrca produced clear, generally round plaques with diameters ranging from 0.1 mm to 0.75 mm. There was some variation with the plaque clarity, with some plaques presenting small bubbles in the center of large plaques, however the rest of the plaque was completely cleared. The plaque shape was also slightly varied with some plaques exhibiting a more oblong shape and many of the plaques exhibiting circular shapes with either a rough (wavy) or smooth outline. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewFidgetOrca_1 FidgetOrca_2 FidgetOrca_3 FidgetOrca_4 FidgetOrca_5 FidgetOrca_6 FidgetOrca_7 FidgetOrca_8 FidgetOrca_9 FidgetOrca_10 FidgetOrca_11 FidgetOrca_12 FidgetOrca_13 FidgetOrca_14 FidgetOrca_15 FidgetOrca_16 FidgetOrca_17 FidgetOrca_18 FidgetOrca_19 FidgetOrca_20 FidgetOrca_21 FidgetOrca_22 FidgetOrca_23 FidgetOrca_24 FidgetOrca_25 FidgetOrca_26 FidgetOrca_27 FidgetOrca_28 FidgetOrca_29 FidgetOrca_30 FidgetOrca_31 FidgetOrca_32 FidgetOrca_33 FidgetOrca_34 FidgetOrca_35 FidgetOrca_36 FidgetOrca_37 FidgetOrca_38 FidgetOrca_39 FidgetOrca_40 FidgetOrca_41 FidgetOrca_42 FidgetOrca_43 FidgetOrca_44 FidgetOrca_45 FidgetOrca_46 FidgetOrca_47 FidgetOrca_48 FidgetOrca_49 FidgetOrca_50 FidgetOrca_51 FidgetOrca_52 FidgetOrca_53 FidgetOrca_54 FidgetOrca_55 FidgetOrca_56 FidgetOrca_57 FidgetOrca_58 FidgetOrca_59 FidgetOrca_60 FidgetOrca_61 FidgetOrca_62 FidgetOrca_63 FidgetOrca_64 FidgetOrca_65 FidgetOrca_66 FidgetOrca_67 FidgetOrca_68 FidgetOrca_69 FidgetOrca_70 FidgetOrca_71 FidgetOrca_72 FidgetOrca_73 FidgetOrca_74 FidgetOrca_75 FidgetOrca_76 FidgetOrca_77 FidgetOrca_78 FidgetOrca_79 FidgetOrca_80 FidgetOrca_81 FidgetOrca_82 FidgetOrca_83 FidgetOrca_84 FidgetOrca_85 FidgetOrca_86 FidgetOrca_87 FidgetOrca_88 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN586056 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2x10^8 pfu/ml |
| Pitt Freezer Box# | 104 |
| Pitt Freezer Box Grid# | B2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
