Streptomyces phage FidgetOrca
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Detailed Information for Phage FidgetOrca
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByAnne Remorca
Year Found2019
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.208929 N, 97.147668 W Map
Discovery NotesThe soil sample was discovered under a tree near a bench located between the UNT's Business Leadership Building and Crumely Hall. The collection site has light foot traffic and has plenty of leaves, acorns, and organisms (such as squirrels and insects). The weather at the time of discovery was rainy, cloudy, and cold. Though at the time of collection it was not raining, the soil sample was still damp from the night before.
Naming NotesThe name for this phage comes from the people who inspire me. The first half of my phage name comes from what my friends and I jokingly call our friend group, the Fidget Friends, and represents all of the times that my friends have helped and supported me. The second half of my phage name is from the second half of my last name, Remorca, to honor my family who support and encourage me to continue to work hard.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 26, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1672
Genome length (bp)56115
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCCGCCT
GC Content59.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBI
SubclusterBI1
Cluster Life CycleLytic
Lysogeny NotesThe particular sample produced a negative result, however the test does not produce a definite result due to the fact that the chosen sample may not be indicative for all the phage's results. In short, though the particular test produced a negative result, a positive result could be possible with a different test sample.
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusBeing Annotated (Expected completion by 12/26/2019)
Plaque NotesTypically FidgetOrca produced clear, generally round plaques with diameters ranging from 0.1 mm to 0.75 mm. There was some variation with the plaque clarity, with some plaques presenting small bubbles in the center of large plaques, however the rest of the plaque was completely cleared. The plaque shape was also slightly varied with some plaques exhibiting a more oblong shape and many of the plaques exhibiting circular shapes with either a rough (wavy) or smooth outline.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2x10^8 pfu/ml
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload