Streptomyces phage ClubPenguin
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Detailed Information for Phage ClubPenguin
Discovery Information
Isolation HostStreptomyces mirabilis NRRL B-2400
Found ByKathryn Wilford, Aarsh Sheth
Year Found2019
Location FoundMillersville, MD United States
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates39.096152 N, 76.573399 W Map
Discovery NotesWas collected on the brim of pond treated with copper sulfate
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2295
Genome length (bp)56205
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCCGCCT
GC Content59.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBI
SubclusterBI7
Cluster Life CycleLytic
Lysogeny NotesAfter some lysogeny testing, we saw some spots form on a plaque growing with the host Scabei. After scratch testing of what might be a mesa, we concluded that the phage was virulent.
Other Cluster Members
Annotating InstitutionUniversity of Maryland, Baltimore County
Annotation StatusIn GenBank
Plaque NotesThe plaques were completely colorless and clear. They were are 2mm, circular in shape with an undefined border. The border was jagged but with an overall very circular looking shape
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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ClubPenguin_1

ClubPenguin_2
(HNH endonuclease)

ClubPenguin_3
(endolysin)

ClubPenguin_4

ClubPenguin_5
(phosphoesterase)

ClubPenguin_6
(terminase large subunit)

ClubPenguin_7
(peptidase)

ClubPenguin_8

ClubPenguin_9

ClubPenguin_10
(portal protein)

ClubPenguin_11

ClubPenguin_12
(major capsid and protease fusion protein)

ClubPenguin_13

ClubPenguin_14
(tail terminator)

ClubPenguin_15
(major tail protein)

ClubPenguin_16

ClubPenguin_17
(major tail protein)

ClubPenguin_18
(tail fiber protein)

ClubPenguin_19

ClubPenguin_20

ClubPenguin_21

ClubPenguin_22
(tape measure protein)

ClubPenguin_23
(minor tail protein)

ClubPenguin_24
(minor tail protein)

ClubPenguin_25

ClubPenguin_26
(hydrolase)

ClubPenguin_27

ClubPenguin_28
(holin)

ClubPenguin_29

ClubPenguin_30

ClubPenguin_31

ClubPenguin_32

ClubPenguin_33

ClubPenguin_34

ClubPenguin_35

ClubPenguin_36

ClubPenguin_37

ClubPenguin_38

ClubPenguin_39

ClubPenguin_40

ClubPenguin_41

ClubPenguin_42
(tellurium resistance protein D family)

ClubPenguin_43

ClubPenguin_44

ClubPenguin_45
(lipoprotein)

ClubPenguin_46

ClubPenguin_47

ClubPenguin_48

ClubPenguin_49
(peptidase)

ClubPenguin_50

ClubPenguin_51

ClubPenguin_52

ClubPenguin_53

ClubPenguin_54

ClubPenguin_55

ClubPenguin_56

ClubPenguin_57

ClubPenguin_58

ClubPenguin_59

ClubPenguin_60

ClubPenguin_61

ClubPenguin_62

ClubPenguin_63
(DNA primase/polymerase)

ClubPenguin_64

ClubPenguin_65

ClubPenguin_66

ClubPenguin_67
(helix-turn-helix DNA binding domain protein)

ClubPenguin_68

ClubPenguin_69

ClubPenguin_70

ClubPenguin_71

ClubPenguin_72
(DprA-like DNA processing chain A)

ClubPenguin_73

ClubPenguin_74

ClubPenguin_75

ClubPenguin_76

ClubPenguin_77

ClubPenguin_78

ClubPenguin_79

ClubPenguin_80

ClubPenguin_81

ClubPenguin_82

ClubPenguin_83

ClubPenguin_84

ClubPenguin_85

ClubPenguin_86
(DNA helicase)

ClubPenguin_87
(HNH endonuclease)

ClubPenguin_88
(nuclease)

ClubPenguin_89
(RecB-like exonuclease/helicase)

ClubPenguin_90
(helix-turn-helix DNA binding domain protein)

ClubPenguin_91

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT310852
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.2e10
Pitt Freezer Box# 101
Pitt Freezer Box Grid# A8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload