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Streptomyces phage Annihilus
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Detailed Information for Phage Annihilus
Discovery Information
Isolation HostStreptomyces scabiei RL-34
Found ByChristian Queen, Mei Zheng
Year Found2021
Location FoundGlen Burnie, MD United States
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature21°C
GPS Coordinates39.133021 N, 76.628615 W Map
Discovery NotesThis phage was discovered in Glen Burnie, Maryland, USA on 09/14/2021 at a temperature of 21.1º C. The soil where the phage was extracted from was moist and grain-like.
Naming NotesAnnihilus is a supervillian from Marvel comics; this was a perfect description of our phage because it annihilated Streptomyces scabiei on our 10^0 from our first round of purification, leaving no trace of the bacteria on the plate.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 3, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage6170
Genome length (bp)43562
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCCCT
GC Content61.2%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Maryland, Baltimore County
Annotation StatusIn GenBank
Plaque NotesPlaque diameter was approximately 2.54 mm; there were no turbidity around the plaque, the plaque was clear and circular.
Number of Genes59
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON081336
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.05 x 10^9 pfu/mL
Pitt Freezer Box# 131
Pitt Freezer Box Grid# G6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload