Microbacterium phage Antares
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Detailed Information for Phage Antares
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found BySumaiya Begum & Meghana Bindiganavale
Year Found2018
Location FoundPhiladelphia, PA USA
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature17°C
GPS Coordinates39.9566 N, 75.1899 W Map
Discovery NotesThe soil sample was dug approximately 12 hours after it had started raining. Therefore, the surrounding environment was moist and muddy. The collection site had no grass. Furthermore, the sample site contained many roots since it was a couple of feet away from trees. The soil sample was collected after digging 3.81 cm down.
Naming NotesAntares is one of the top 20 most brightest stars visible in the night sky. Antares is a notable slow irregular variable star due to its dynamic periodicity with light emissions. Therefore, this phage was named Antares because it showed varying plaque sizes before multiple rounds of purification.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage57
Genome length (bp)53362
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEC
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionDrexel University
Annotation StatusIn GenBank
Plaque NotesThe clear plaques indicated that the phages were in the lytic cycle, and they had lysed all of the host bacteria. The smallest plaque had a diameter of 0.5 mm and the largest plaque had a diameter of 1.0 mm. The plaques were dispersed all around the plate. Additionally, the plaques were smooth around the edges.
Has been Phamerated?Yes
Gene List

Click to View

Antares_1
(terminase, small subunit)

Antares_2

Antares_3

Antares_4
(terminase, large subunit)

Antares_5

Antares_6

Antares_7

Antares_8

Antares_9

Antares_10

Antares_11

Antares_12

Antares_13

Antares_14

Antares_15

Antares_16

Antares_17

Antares_18

Antares_19

Antares_20
(portal protein)

Antares_21

Antares_22

Antares_23
(MuF-like minor capsid protein)

Antares_24

Antares_25
(capsid maturation protease)

Antares_26

Antares_27

Antares_28

Antares_29

Antares_30

Antares_31
(portal protein)

Antares_32

Antares_33

Antares_34
(tail assembly chaperone)

Antares_35
(tail assembly chaperone)

Antares_36
(tape measure protein)

Antares_37
(minor tail protein)

Antares_38
(minor tail protein)

Antares_39
(minor tail protein)

Antares_40
(minor tail protein)

Antares_41

Antares_42

Antares_43
(lysin A)

Antares_44
(membrane protein)

Antares_45

Antares_46
(membrane protein)

Antares_47

Antares_48
(exonuclease)

Antares_49
(ssDNA binding protein, ERF family)

Antares_50

Antares_51

Antares_52
(helix-turn-helix DNA binding domain)

Antares_53
(ADP-ribosyltransferase)

Antares_54
(HNH endonuclease)

Antares_55

Antares_56

Antares_57

Antares_58

Antares_59

Antares_60

Antares_61

Antares_62

Antares_63

Antares_64
(ssDNA binding protein)

Antares_65

Antares_66

Antares_67
(helix-turn-helix DNA binding domain)

Antares_68

Antares_69

Antares_70

Antares_71

Antares_72

Antares_73

Antares_74

Antares_75

Antares_76

Antares_77

Antares_78

Antares_79

Antares_80

Antares_81
(membrane protein)

Antares_82
(RNA polymerase sigma factor)

Antares_83
(ParB-like nuclease domain)

Antares_84

Antares_85
(glycosyltransferase)

Antares_86
(UDP-glucose dehydrogenase)

Antares_87
(AAA-ATPase)

Antares_88

Antares_89

Antares_90

Antares_91

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN444877
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 81
Pitt Freezer Box Grid# H3
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload