Microbacterium phage Metamorphoo
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Detailed Information for Phage Metamorphoo
Discovery Information
Isolation HostMicrobacterium paraoxydans NWU1
Found ByLaurel M. Heskett
Year Found2015
Location FoundOmaha, NE USA
Finding InstitutionNebraska Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates41.195397 N, 96.162099 W Map
Discovery NotesThe soil that contained the phage sample was obtained under a deck where the soil was exposed and moist compared to the soil that produced grass around the deck. There was a worm in the soil collected from a depth of 3 inches.
Naming NotesThe name Metamorphoo means transformation in Greek. At first the phage appeared to be temperate, but the second spot test revealed that the phage was lytic and it continued to appear lytic throughout the rest of the tame process. This seems to be a visual transformation.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 27, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage264
Genome length (bp)54148
Character of genome endsUnknown
GC Content68.5%
Sequencing NotesAppears to be circularly permuted, but not verified. Appears to have predominantly single-strand coverage.
Fasta file available?Yes: Download fasta file
Characterization
ClusterEC
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNebraska Wesleyan University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Metamorphoo_1
(terminase small subunit)

Metamorphoo_2

Metamorphoo_3
(terminase large subunit)

Metamorphoo_4

Metamorphoo_5

Metamorphoo_6

Metamorphoo_7

Metamorphoo_8

Metamorphoo_9

Metamorphoo_10

Metamorphoo_11

Metamorphoo_12

Metamorphoo_13

Metamorphoo_14

Metamorphoo_15

Metamorphoo_16

Metamorphoo_17

Metamorphoo_18

Metamorphoo_19

Metamorphoo_20
(portal protein)

Metamorphoo_21

Metamorphoo_22

Metamorphoo_23
(MuF-like minor capsid protein)

Metamorphoo_24

Metamorphoo_25
(capsid maturation protease)

Metamorphoo_26

Metamorphoo_27

Metamorphoo_28

Metamorphoo_29

Metamorphoo_30

Metamorphoo_31

Metamorphoo_32

Metamorphoo_33

Metamorphoo_34

Metamorphoo_93

Metamorphoo_36
(tape measure protein)

Metamorphoo_37
(minor tail protein)

Metamorphoo_38
(minor tail protein)

Metamorphoo_39
(minor tail protein)

Metamorphoo_40
(minor tail protein)

Metamorphoo_41

Metamorphoo_42

Metamorphoo_43

Metamorphoo_44
(lysin A)

Metamorphoo_45
(membrane protein)

Metamorphoo_46
(membrane protein)

Metamorphoo_47
(membrane protein)

Metamorphoo_48

Metamorphoo_49
(resolvase)

Metamorphoo_50
(exonuclease)

Metamorphoo_51
(ERF family ssDNA binding protein)

Metamorphoo_52

Metamorphoo_53

Metamorphoo_54

Metamorphoo_55

Metamorphoo_56
(HNH endonuclease)

Metamorphoo_57

Metamorphoo_58

Metamorphoo_59

Metamorphoo_60

Metamorphoo_61

Metamorphoo_94

Metamorphoo_62

Metamorphoo_63

Metamorphoo_64
(DNA ligase)

Metamorphoo_65

Metamorphoo_66

Metamorphoo_67
(ssDNA binding protein)

Metamorphoo_68
(membrane protein)

Metamorphoo_69
(DNA binding protein)

Metamorphoo_70
(DNA binding protein)

Metamorphoo_71

Metamorphoo_72

Metamorphoo_73

Metamorphoo_74

Metamorphoo_75

Metamorphoo_76

Metamorphoo_77

Metamorphoo_78
(RNA polymerase sigma factor)

Metamorphoo_79

Metamorphoo_80

Metamorphoo_81

Metamorphoo_82

Metamorphoo_83

Metamorphoo_84
(ParB-like nuclease domain protein)

Metamorphoo_85
(glycosyltransferase)

Metamorphoo_86
(UDP-glucose dehydrogenase)

Metamorphoo_87
(AAA-ATPase)

Metamorphoo_88

Metamorphoo_89

Metamorphoo_90

Metamorphoo_91

Metamorphoo_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH271304
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.8*10^10
Pitt Freezer Box# 27
Pitt Freezer Box Grid# B5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload