| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hillary Sigale and Sarah Jacobs |
| Year Found | 2010 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.645638 N, 90.311388 W Map |
| Discovery Notes | Soil moist but not saturated, soil from base of compost bins in Burning Kumquat organic garden on Washington University's campus, air temperature was approximately 29C, sample taken from top soil (about 1-2 cm deep). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2012 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 136 |
| Sanger Finishing Reactions | 3 |
| Genome length (bp) | 41893 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Collin College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and circular with sharp distinct edges. Plaque diameter is approximately .10-.15 cm. |
| Morphotype | Siphoviridae |
| Number of Genes | 61 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK305884 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
WUSL |
| Pitt Freezer Box# |
20 |
| Pitt Freezer Box Grid# |
24 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
