Mycobacterium phage Annihilator
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Detailed Information for Phage Annihilator
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLaura Watkins and Jeffrey Wei
Year Found2011
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.6467 N, 90.2958 W Map
Discovery NotesMoist soil, from a tree stump, 75 Degrees Fahrenheit, 9/5/2011
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 20, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage406
Sanger Finishing Reactions2
Genome length (bp)41893
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall to medium sized plaques, Mostly clear, little variation in turbidity or size
Number of Genes62
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT365399
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2011WUSLannihilatorKH
SEA Lysate Titer 2.18e11
Date of SEA Lysate Titering Sep 29, 2011
Pitt Freezer Box# 31
Pitt Freezer Box Grid# 29
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload