| Detailed Information for Phage Annihilator |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Laura Watkins and Jeffrey Wei |
| Year Found | 2011 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.6467 N, 90.2958 W Map |
| Discovery Notes | Moist soil, from a tree stump, 75 Degrees Fahrenheit, 9/5/2011 |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 20, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 406 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 41893 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small to medium sized plaques, Mostly clear, little variation in turbidity or size |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT365399 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLannihilatorKH |
| SEA Lysate Titer |
2.18e11 |
| Date of SEA Lysate Titering |
Sep 29, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
29 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |