Mycobacterium phage Marmie
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Detailed Information for Phage Marmie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMeliya Hart
Year Found2021
Location FoundPhiladelphia, PA United States
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature3°C
GPS Coordinates39.946662 N, 75.204501 W Map
Discovery NotesSoil was moist and was collected from 3 centimeters deep. It was found next to (about five feet away) an old storage building in the cemetery. Snow melted in the area the sample was taken, but the surrounding area had about a quarter inch of snow. Ground is slightly sloped and the area sample was taken from was closer to the top of the slope. There is minimal foot traffic because it is off of the paved walking path. There are several trees around, the closest being about ten feet away. There was a second house about 200 feet away and a garden area about 50 feet away.
Naming NotesNamed after my dog Marmie since my two other dogs' names, Samson and Primrose, were taken.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 28, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1472
Genome length (bp)41902
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusIn GenBank
Plaque NotesThe plaque size ranged from between 1/2 millimeter to 1 millimeter. Plaques are clear and round making this Phage a lytic phage.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON637766
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.3x10^9
Pitt Freezer Box# 130
Pitt Freezer Box Grid# C3
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload