Mycobacterium phage Phreak
Phreak EM Picture
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Detailed Information for Phage Phreak
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPetros Raygoza
Year Found2012
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates47.66709, -117.40304 Map
Discovery Notesfrom a flowerbed, sunny and 80 degrees Fahrenheit
Naming NotesNamed after SF Giants pitcher Tim Lincecum, "The Freak".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage460
Sanger Finishing Reactions8
Genome length (bp)41901
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members

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Angel (G1)

Annihilator (G1)

Aroostook (G1)

Avrafan (G1)

Barkley26 (G1)

BPs (G1)

BQuat (G1)

BruceB (G1)

Camri (G1)

CassieYates (G1)

Cedasite (G1)

Chance64 (G1)

Cherrybomb426 (G1)

CLED96 (G1)

Coleslaw (G1)

Crespo (G1)

Darionha (G1)

DMoney (G1)

ECartman (G1)

Frosty24 (G1)

Gideon (G1)

GoldenAsh (G1)

Gomashi (G1)

Grizzly (G1)

Halo (G1)

Hope (G1)

Hotshotbaby7 (G1)

Jane (G1)

Jolene (G1)

Jonghyun (G1)

JorRay (G1)

Kareem (G1)

Kasen3 (G1)

Liefie (G1)

LouisV14 (G1)

Marmie (G1)

Mowgli (G1)

OctaviousRex (G1)

Olga (G1)

Paito (G1)

Peeb (G1)

Periodt (G1)

Phish (G1)

Phreak (G1)

PinkYoshi (G1)

Plagueis (G1)

Rabbs (G1)

Remy19 (G1)

Renaissance (G1)

Schiebel (G1)

ShaboiShabazz (G1)

Sizemore (G1)

Sneeze (G1)

Sweets (G1)

Taheera (G1)

Terror (G1)

TomBrady (G1)

ZoMa (G1)

Zombie (G1)

Avocado (G2)

Cambiare (G2)

FlagStaff (G2)

Pace1224 (G2)

MOOREtheMARYer (G3)

Pinnie (G3)

Jolie2 (G4)

Lemuria (G4)

Mercurio (G4)

Antsirabe (G5)

Stargaze (G5)
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Phreak_1

Phreak_2
(terminase)

Phreak_3
(portal protein)

Phreak_4
(capsid maturation protease)

Phreak_5

Phreak_6
(scaffolding protein)

Phreak_7
(major capsid protein)

Phreak_8
(head-to-tail connector)

Phreak_9
(head-to-tail adaptor)

Phreak_10
(head-to-tail stopper)

Phreak_11

Phreak_12
(tail terminator)

Phreak_13
(major tail protein)

Phreak_14
(tail assembly chaperone)

Phreak_15
(tail assembly chaperone)

Phreak_16
(tape measure protein)

Phreak_17
(minor tail protein)

Phreak_18
(minor tail protein)

Phreak_19
(minor tail protein)

Phreak_20
(minor tail protein)

Phreak_21
(minor tail protein)

Phreak_22
(minor tail protein)

Phreak_23

Phreak_24

Phreak_25

Phreak_26

Phreak_27
(lysin A)

Phreak_28
(lysin B)

Phreak_29
(holin)

Phreak_30

Phreak_31

Phreak_32
(integrase)

Phreak_33
(immunity repressor)

Phreak_34
(recombination directionality factor)

Phreak_35

Phreak_36

Phreak_37

Phreak_38

Phreak_39

Phreak_40

Phreak_41

Phreak_42
(exonuclease)

Phreak_43
(ssDNA binding protein)

Phreak_44

Phreak_45

Phreak_46

Phreak_47

Phreak_48

Phreak_49

Phreak_50

Phreak_51
(RuvC-like resolvase)

Phreak_52

Phreak_53
(MPME1 protein)

Phreak_54

Phreak_55

Phreak_56

Phreak_57

Phreak_58

Phreak_59

Phreak_60

Phreak_61

Phreak_62
(HNH endonuclease)

Phreak_63
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT347314
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 36
Pitt Freezer Box Grid# 8
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload