Mycobacterium phage Jolene
Jolene EM Picture
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Detailed Information for Phage Jolene
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAsli Ozdemir
Year Found2020
Location FoundLas Vegas, NV United States of America
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature21°C
GPS Coordinates36.109722 N, 115.1425 W Map
Discovery NotesThe sample was collected from a sample of Miracle Grow Moisture Control Potting Mix. The soil was dry, granular, dark brown, and sediment rich. It was collected at 36° 6' 35" N, 115° 8' 33" W at an elevation of 612 meters with a 5% humidity index and a temperature of 21°C.
Naming NotesNamed after my sister's favorite Dolly Parton song.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 22, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage156
Genome length (bp)42305
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.7%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Nevada Las Vegas
Annotation StatusIn GenBank
Plaque NotesThe plaques ranged from 2 mm to 2.75 mm in diameter. They were all symmetrical, round, clear, and smooth. All plaques were consistent in shape and size.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ171377
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 120
Pitt Freezer Box Grid# H5
Available Files
GenBank File for PhameratorDownload