The Actinobacteriophage Database

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Detailed Information for Phage Frosty24
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Reshard McElrath
Year Found	2011
Location Found	Atlanta, GA USA
Finding Institution	Morehouse College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	33.747650 N, 84.415900 W Map
Discovery Notes	Area that contains plants beside a greenhouse. Soil was taken from the surface.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 26, 2012
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Ion Torrent Sequencing
Approximate Shotgun Coverage	85
Genome length (bp)	41901
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CCCCATGGCAT
GC Content	66.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	G
Subcluster	G1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Angel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Remy19 (G1) Renaissance (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TomBrady (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) Pace1224 (G2) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Extremely lysed. Small plaque.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Frosty24_1 (terminase small subunit) Frosty24_2 (terminase large subunit) Frosty24_3 (portal protein) Frosty24_4 (capsid maturation protease) Frosty24_5 Frosty24_6 (scaffolding protein) Frosty24_7 (major capsid protein) Frosty24_8 (head-to-tail connector protein) Frosty24_9 (head-to-tail adaptor) Frosty24_10 (head-to-tail stopper) Frosty24_11 Frosty24_12 (tail terminator) Frosty24_13 (major tail protein) Frosty24_14 (tail assembly chaperone) Frosty24_15 (tail assembly chaperone) Frosty24_16 (tape measure protein) Frosty24_17 Frosty24_18 (minor tail protein) Frosty24_19 Frosty24_20 (minor tail protein) Frosty24_21 (minor tail protein) Frosty24_22 (minor tail protein) Frosty24_23 Frosty24_24 Frosty24_25 Frosty24_26 Frosty24_27 (lysin A) Frosty24_28 (lysin B) Frosty24_29 (holin) Frosty24_30 Frosty24_31 Frosty24_32 (integrase) Frosty24_33 (immunity repressor) Frosty24_34 (excise) Frosty24_35 Frosty24_36 Frosty24_37 Frosty24_38 (ASC-1-like domain protein) Frosty24_39 Frosty24_40 Frosty24_41 Frosty24_42 (exonuclease) Frosty24_43 (ssDNA binding protein) Frosty24_44 Frosty24_45 Frosty24_46 Frosty24_47 Frosty24_48 Frosty24_49 Frosty24_50 Frosty24_51 (RuvC-like resolvase) Frosty24_52 Frosty24_53 Frosty24_54 Frosty24_55 Frosty24_56 Frosty24_57 Frosty24_58 Frosty24_59 Frosty24_60 Frosty24_61 Frosty24_62 (HNH endonuclease) Frosty24_63
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KT355474
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	Dr. Piester
Pitt Freezer Box#	26
Pitt Freezer Box Grid#	24
Available Files
Plaque Picture	Download
Final DNAMaster File	Download

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