Mycobacterium phage Gomashi
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Detailed Information for Phage Gomashi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesBlossom
Found ByDaphne Hussey
Year Found2012
Location FoundRiver Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.851325 N, 92.614663 W Map
Discovery NotesI found my soil outside of Ames Suites on the UWRF campus. The area where I found it was low traffic and in the shade. I dug about 4-5 inches into the dirt where it was cool and moist.
Naming NotesMy phage was super small so I named it Gomashi, which is a Japanese word meaning "little sesame seed". I felt this fit my phage because of their size. Its also the name of my cat.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 7, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage360
Sanger Finishing Reactions4
Genome length (bp)41903
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesWhen we first started this process my plaque were a little larger and had halos around them. However, after we isolated them I found that they were really small, and there were a lot of them.
Number of Genes61
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM923970
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^11
Date of SEA Lysate Titering Oct 15, 2012
Pitt Freezer Box# 41
Pitt Freezer Box Grid# 40