Mycobacterium phage TomBrady
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Detailed Information for Phage TomBrady
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesTB12
Found ByChandra Tupurani and Kyle Stoecker
Year Found2021
Location FoundRichmond, VA United States
Finding InstitutionVirginia Commonwealth University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.548472 N, 77.45375 W Map
Discovery NotesFound in dirt underneath some mulch.
Naming NotesWhile we were working on growing isolated plaques for the phage that we found, many struggles were encountered with pipetting. But in the end, we ended up with excellent TEM images. So despite all the struggles, we ended up where we were supposed to, so this phage was named after the comeback kid himself.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3187
Genome length (bp)41902
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Commonwealth University
Annotation StatusIn GenBank
Plaque NotesVery clustered, looked like lots of fish eggs in a cluster.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON970618
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 145
Pitt Freezer Box Grid# E10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload