| Detailed Information for Phage Beelzebub |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kristina Sevcik |
| Year Found | 2017 |
| Location Found | Lincoln, NE USA |
| Finding Institution | Northwestern College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.76289 N, 96.62133 W Map |
| Discovery Notes | The phage was discovered by Patrick Wagner and adopted from an enrichment isolation by Kristina Sevcik. The soil came from a garden next to a compost pile. |
| Naming Notes | The phage was named Beelzebub because at many times it was a devil to work with. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 2, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 636 |
| Genome length (bp) | 65529 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Northwestern College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology was typically small, clear plaques with a slight halo. |
| Morphotype | Siphoviridae |
| Number of Genes | 118 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK061407 |
| Refseq Number | None yet |
| Published in Paper | Sevcik et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Cluster S Mycobacteriophages Beelzebub, Raela, and RedRaider77 |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.3x10^11 |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
