Mycobacterium phage Blackbeetle
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Detailed Information for Phage Blackbeetle
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMakayla Sharelle Richard, Gabrielle Nadolny
Year Found2016
Location FoundMelrose, MA United States
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates4.8 N, 34.8 E Map
Discovery NotesThis soil sample was taken from a potted tomato plant. The soil is bagged soil purchased from a garden store. The plant was watered daily all summer and was kept in a sunny area.
Naming NotesWell, while making this experiment the song Black Beetles came out and my lab partners and I because we were always singing it and it always got tuck in our head so we decided to name our little phage baby Blackbeetle.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1353
Genome length (bp)64824
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterS
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesOur plaque was about 1mm in length and 2 mm in width. It was a halo like shape and very tiny.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Blackbeetle_1

Blackbeetle_2

Blackbeetle_3

Blackbeetle_4

Blackbeetle_5

Blackbeetle_6

Blackbeetle_7

Blackbeetle_8

Blackbeetle_9

Blackbeetle_10

Blackbeetle_11

Blackbeetle_12

Blackbeetle_13

Blackbeetle_14

Blackbeetle_15

Blackbeetle_16

Blackbeetle_17

Blackbeetle_18

Blackbeetle_19

Blackbeetle_20

Blackbeetle_21

Blackbeetle_22

Blackbeetle_23

Blackbeetle_24
(helix-turn-helix DNA binding domain)

Blackbeetle_25

Blackbeetle_26

Blackbeetle_27

Blackbeetle_28

Blackbeetle_29

Blackbeetle_30

Blackbeetle_31

Blackbeetle_32
(methyltransferase)

Blackbeetle_33

Blackbeetle_34

Blackbeetle_35
(terminase)

Blackbeetle_36
(portal protein)

Blackbeetle_37
(capsid maturation protease)

Blackbeetle_38
(scaffolding protein)

Blackbeetle_39
(capsid decoration protein)

Blackbeetle_40
(major capsid protein)

Blackbeetle_41
(head-to-tail adaptor)

Blackbeetle_42
(head-to-tail stopper)

Blackbeetle_43

Blackbeetle_44
(tail terminator)

Blackbeetle_45
(major tail protein)

Blackbeetle_46
(tail assembly chaperone)

Blackbeetle_47
(tail assembly chaperone)

Blackbeetle_48
(tape measure protein)

Blackbeetle_49
(minor tail protein)

Blackbeetle_50
(minor tail protein)

Blackbeetle_51
(HNH endonuclease)

Blackbeetle_52

Blackbeetle_53

Blackbeetle_54
(lysin A)

Blackbeetle_55

Blackbeetle_56
(lysin B)

Blackbeetle_57

Blackbeetle_58

Blackbeetle_59

Blackbeetle_60

Blackbeetle_61

Blackbeetle_62
(helix-turn-helix DNA binding domain, MerR-like)

Blackbeetle_63

Blackbeetle_64

Blackbeetle_65

Blackbeetle_66
(exonuclease)

Blackbeetle_67

Blackbeetle_68

Blackbeetle_69
(HNH endonuclease)

Blackbeetle_70

Blackbeetle_71

Blackbeetle_72

Blackbeetle_73

Blackbeetle_74
(DprA-like DNA processing chain A)

Blackbeetle_75

Blackbeetle_76

Blackbeetle_77

Blackbeetle_78

Blackbeetle_79

Blackbeetle_80

Blackbeetle_81

Blackbeetle_82

Blackbeetle_83

Blackbeetle_84

Blackbeetle_85
(O-methyltransferase)

Blackbeetle_86

Blackbeetle_87
(galactosyltransferase)

Blackbeetle_88

Blackbeetle_89
(glycosyltransferase)

Blackbeetle_90

Blackbeetle_91

Blackbeetle_92

Blackbeetle_93
(HNH endonuclease)

Blackbeetle_94
(minor tail protein, D-ala-D-ala carboxypeptidase)

Blackbeetle_95
(minor tail protein)

Blackbeetle_96

Blackbeetle_97

Blackbeetle_98

Blackbeetle_99

Blackbeetle_100

Blackbeetle_101

Blackbeetle_102

Blackbeetle_103

Blackbeetle_104

Blackbeetle_105

Blackbeetle_106

Blackbeetle_107

Blackbeetle_108

Blackbeetle_109

Blackbeetle_110

Blackbeetle_111

Blackbeetle_112

Blackbeetle_113

Blackbeetle_114
(glutamine amidotransferase domain)

Blackbeetle_115

Blackbeetle_116

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494123
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 73
Pitt Freezer Box Grid# G11
Available Files
GenBank File for PhameratorDownload