Detailed Information for Phage Lilbit |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Kate Aberger |
Year Found | 2014 |
Location Found | New Haven, CT USA |
Finding Institution | Southern Connecticut State University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 42°C |
GPS Coordinates | 41.0 N, 72.0 W Map |
Discovery Notes | Found in 3 inches deep in very most soil in a compost pile The temperature was 64 degrees F and it rained over the weekend Lots of insects could live at the location that I found it Grass, leaves, tree brances, and dirt surround the site of discovery Discovered on 9-22-14 Ave capsid width (nm) = 69.81 +/- 3.13 Ave tail length (nm) = 283.58 +/- 7.02 |
Naming Notes | I named this phage "Lilbit" because it creates tiny plaques with a diameter of 1 mm |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Nov 7, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 419 |
Genome length (bp) | 65106 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | GCGCGCAGCGC |
GC Content | 63.4% |
Sequencing Notes | This phage was identified as being uncommon during a large DOGEMS screening run, then subsequently grown from frozen stock and sequenced. |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | S |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Southern Connecticut State University |
Annotation Status | Overdue (Expected completion by 12/15/2023) |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2014SCSUlilbitKA |
SEA Lysate Titer |
2.5 x 10^10 |
Date of SEA Lysate Titering |
Oct 21, 2014 |
Pitt Freezer Box# |
10 |
Pitt Freezer Box Grid# |
B7 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |