Mycobacterium phage Lilbit
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Detailed Information for Phage Lilbit
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKate Aberger
Year Found2014
Location FoundNew Haven, CT USA
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature42°C
GPS Coordinates41.0 N, 72.0 W Map
Discovery NotesFound in 3 inches deep in very most soil in a compost pile
The temperature was 64 degrees F and it rained over the weekend
Lots of insects could live at the location that I found it
Grass, leaves, tree brances, and dirt surround the site of discovery
Discovered on 9-22-14
Ave capsid width (nm) = 69.81 +/- 3.13
Ave tail length (nm) = 283.58 +/- 7.02
Naming NotesI named this phage "Lilbit" because it creates tiny plaques with a diameter of 1 mm
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage419
Genome length (bp)65106
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Sequencing NotesThis phage was identified as being uncommon during a large DOGEMS screening run, then subsequently grown from frozen stock and sequenced.
Fasta file available?Yes: Download fasta file
Characterization
ClusterS
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionFlorida International University
Annotation StatusOverdue (Expected completion by 5/15/2020)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2014SCSUlilbitKA
SEA Lysate Titer 2.5 x 10^10
Date of SEA Lysate Titering Oct 21, 2014
Pitt Freezer Box# 10
Pitt Freezer Box Grid# B7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload