Mycobacterium phage Poise
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Detailed Information for Phage Poise
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesPayge
Found ByCatherine Rautio, Julianna DeSantis, Lisa Nguyenho
Year Found2016
Location FoundMelrose, MA USA
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.451333 N, 71.076333 W Map
Discovery NotesComing from a beautiful tomato plants soil, our phage was discovered among the damp bottom of the pot, that was watered every day. Seemingly we thought it would be a disaster taking soil that was store bought for the growing of a plant, however we were in for a surprise. It turned out to be a great success, and has given us nothing but hope for the long lasting strong phage!
Naming NotesOur phage seemed like a victory due to having a rare 3 phage DNA and our titer being extremely high, therefore our original name being "Lil Jackpot". With much thought, we decided Paige was much cuter.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1816
Genome length (bp)64824
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterS
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionMerrimack College
Annotation StatusQC Complete
Plaque NotesPlaques grew very slow and seemed to be turbid when done. As well as being small.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Poise_1

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Poise_7

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Poise_9

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Poise_12

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Poise_14

Poise_15

Poise_16

Poise_17

Poise_18

Poise_19

Poise_20

Poise_21

Poise_22

Poise_23
(membrane protein)

Poise_24
(helix-turn-helix DNA binding domain)

Poise_25

Poise_26

Poise_27

Poise_28

Poise_29

Poise_30

Poise_31

Poise_32
(DNA methylase)

Poise_33

Poise_34

Poise_35
(terminase)

Poise_36
(portal protein)

Poise_37
(MuF-like minor capsid protein)

Poise_38
(scaffolding protein)

Poise_39
(capsid decoration protein)

Poise_40
(major capsid protein)

Poise_41
(head-to-tail adaptor)

Poise_42

Poise_43

Poise_44
(tail terminator)

Poise_45
(major tail protein)

Poise_46
(tail assembly chaperone)

Poise_47
(tail assembly chaperone)

Poise_48
(tape measure protein)

Poise_49
(minor tail protein)

Poise_50
(minor tail protein)

Poise_51
(HNH endonuclease)

Poise_52

Poise_53

Poise_54
(lysin A)

Poise_55

Poise_56
(lysin B)

Poise_57
(holin)

Poise_58

Poise_59
(membrane protein)

Poise_60
(membrane protein)

Poise_61
(hydrolase)

Poise_62
(helix-turn-helix DNA binding domain, MerR-like)

Poise_63

Poise_64
(membrane protein)

Poise_65
(helix-turn-helix DNA binding domain)

Poise_66
(exonuclease)

Poise_67

Poise_68

Poise_69
(HNH endonuclease)

Poise_70

Poise_71

Poise_72

Poise_73

Poise_74
(DprA-like DNA processing chain A)

Poise_75

Poise_76

Poise_77

Poise_78

Poise_79

Poise_80
(membrane protein)

Poise_81

Poise_82

Poise_83

Poise_84

Poise_85
(O-methyltransferase)

Poise_86
(glycosyltransferase)

Poise_87
(galactosyltransferase)

Poise_88

Poise_89
(glycosyltransferase)

Poise_90

Poise_91

Poise_92

Poise_93
(HNH endonuclease)

Poise_94
(minor tail protein)

Poise_95
(minor tail protein)

Poise_96

Poise_97

Poise_98

Poise_99

Poise_100
(membrane protein)

Poise_101

Poise_102

Poise_103

Poise_104

Poise_105
(membrane protein)

Poise_106

Poise_107

Poise_108

Poise_109
(membrane protein)

Poise_110

Poise_111

Poise_112

Poise_113

Poise_114
(glutamine amidotransferase domain)

Poise_115

Poise_116

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.8x10^10
Pitt Freezer Box# 73
Pitt Freezer Box Grid# H3
Available Files
GenBank File for PhameratorDownload