Mycobacterium phage Poise
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Detailed Information for Phage Poise
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesPayge
Found ByCatherine Rautio, Julianna DeSantis, Lisa Nguyenho
Year Found2016
Location FoundMelrose, MA USA
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.451333 N, 71.076333 W Map
Discovery NotesComing from a beautiful tomato plants soil, our phage was discovered among the damp bottom of the pot, that was watered every day. Seemingly we thought it would be a disaster taking soil that was store bought for the growing of a plant, however we were in for a surprise. It turned out to be a great success, and has given us nothing but hope for the long lasting strong phage!
Naming NotesOur phage seemed like a victory due to having a rare 3 phage DNA and our titer being extremely high, therefore our original name being "Lil Jackpot". With much thought, we decided Paige was much cuter.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1816
Genome length (bp)64824
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionMerrimack College
Annotation StatusAwaiting_QC
Plaque NotesPlaques grew very slow and seemed to be turbid when done. As well as being small.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.8x10^10
Pitt Freezer Box# 73
Pitt Freezer Box Grid# H3