| Detailed Information for Phage Poise |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Payge |
| Found By | Catherine Rautio, Julianna DeSantis, Lisa Nguyenho |
| Year Found | 2016 |
| Location Found | Melrose, MA USA |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.451333 N, 71.076333 W Map |
| Discovery Notes | Coming from a beautiful tomato plants soil, our phage was discovered among the damp bottom of the pot, that was watered every day. Seemingly we thought it would be a disaster taking soil that was store bought for the growing of a plant, however we were in for a surprise. It turned out to be a great success, and has given us nothing but hope for the long lasting strong phage! |
| Naming Notes | Our phage seemed like a victory due to having a rare 3 phage DNA and our titer being extremely high, therefore our original name being "Lil Jackpot". With much thought, we decided Paige was much cuter. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1816 |
| Genome length (bp) | 64824 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Merrimack College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques grew very slow and seemed to be turbid when done. As well as being small. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN329676 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.8x10^10 |
| Pitt Freezer Box# |
73 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| GenBank File for Phamerator | Download |