| Detailed Information for Phage Bilo | |
| Discovery Information | |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Isabel Shrestha and Hallie Wikfors |
| Year Found | 2020 |
| Location Found | St. Louis, MO United States |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.648946 N, 90.306647 W Map |
| Discovery Notes | It was a soil sample collected near a tree in front of the Cupples II building on Washington University’s Danforth campus near Throop Drive. The weather was sunny, but a little shaded in the area of collection due to the tree’s leaves, and the ambient temperature was approximately 77 degrees on September 26, 2020. The sample consisted of slightly wet soil, full of organic matter, and was gathered from approximately 2 inches below the surface. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 11, 2021 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 263 |
| Genome length (bp) | 43835 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 60.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewCherryBlossom (BI1) DrGrey (BI1) Esketit (BI1) FidgetOrca (BI1) FrodoSwaggins (BI1) GirlPower (BI1) Hoshi (BI1) IceWarrior (BI1) Indigenous (BI1) Jaylociraptor (BI1) Madamato (BI1) Maya (BI1) Meibysrarus (BI1) Namo (BI1) OlympicHelado (BI1) Popy (BI1) Rima (BI1) Soshi (BI1) Spectropatronm (BI1) TaidaOne (BI1) TieDye (BI1) TonyStarch (BI1) Annihilus (BI2) Bilo (BI2) Doxi13 (BI2) GoblinVoyage (BI2) HotFries (BI2) Moozy (BI2) PherryCruz (BI2) RavenPuff (BI2) RosaAsantewaa (BI2) Scap1 (BI2) SheRa (BI2) LibertyBell (BI3) CricKo (BI4) FromIndy (BI4) Miek (BI4) Rainydai (BI4) Regigigas (BI4) SendItCS (BI4) Thiqqums (BI4) Bing (BI5) Kardashian (BI6) ClubPenguin (BI7) |
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques themselves varied in size, ranging from tiny circles to more prevalent ones, but had similar shapes in being circular, although some were slightly jagged around the edges with a more elongated oval shape. However, the majority of the plaques were circular. The plaques were also clear and not cloudy in their appearance. |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBilo_1 Bilo_2 Bilo_3 Bilo_4 Bilo_5 Bilo_6 Bilo_7 Bilo_8 Bilo_9 Bilo_10 Bilo_11 Bilo_12 Bilo_13 Bilo_14 Bilo_15 Bilo_16 Bilo_17 Bilo_18 Bilo_19 Bilo_20 Bilo_21 Bilo_22 Bilo_23 Bilo_24 Bilo_25 Bilo_26 Bilo_27 Bilo_28 Bilo_29 Bilo_30 Bilo_31 Bilo_32 Bilo_33 Bilo_34 Bilo_35 Bilo_36 Bilo_37 Bilo_38 Bilo_39 Bilo_40 Bilo_41 Bilo_42 Bilo_43 Bilo_44 Bilo_45 Bilo_46 Bilo_47 Bilo_48 Bilo_49 Bilo_50 Bilo_51 Bilo_52 Bilo_53 Bilo_54 Bilo_55 Bilo_56 Bilo_57 Bilo_58 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ820086 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |