| Detailed Information for Phage Blackbeetle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Makayla Sharelle Richard, Gabrielle Nadolny |
| Year Found | 2016 |
| Location Found | Melrose, MA USA |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.447410,, -71.076466 Map |
| Discovery Notes | This soil sample was taken from a potted tomato plant. The soil is bagged soil purchased from a garden store. The plant was watered daily all summer and was kept in a sunny area. |
| Naming Notes | Well, while making this experiment the song Black Beetles came out and my lab partners and I because we were always singing it and it always got tuck in our head so we decided to name our little phage baby Blackbeetle. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1353 |
| Genome length (bp) | 64824 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Merrimack College |
| Annotation Status | In GenBank |
| Plaque Notes | Our plaque was about 1mm in length and 2 mm in width. It was a halo like shape and very tiny. |
| Morphotype | Siphoviridae |
| Number of Genes | 116 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494123 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
73 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| GenBank File for Phamerator | Download |