The Actinobacteriophage Database

Mycobacterium phage BruceB

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Detailed Information for Phage BruceB
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Catherine Halpern
Year Found	2012
Location Found	Richmond, VA
Finding Institution	Virginia Commonwealth University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	37.546389 N, 77.454722 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Feb 4, 2013
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454
Sequencer Used	454 Genome Sequencer FLX
Approximate Shotgun Coverage	320
Sanger Finishing Reactions	6
Genome length (bp)	41901
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CCCCATGGCAT
GC Content	66.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	G
Subcluster	G1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Jeilious (None) Angel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) AzulaCat (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DingDing (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Maliketh (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phloodle (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Rattrick (G1) Remy19 (G1) Renaissance (G1) Ruriko (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TinaBug (G1) TomBrady (G1) Wendigo (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) GodSpeed (G2) Pace1224 (G2) Cloudyecho (G3) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5) YangYin (G5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	63
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View BruceB_1 (terminase small subunit) BruceB_2 (terminase large subunit) BruceB_3 (portal protein) BruceB_4 (capsid maturation protease) BruceB_5 BruceB_6 (scaffolding protein) BruceB_7 (major capsid protein) BruceB_8 (head-to-tail connector protein) BruceB_9 (head-to-tail adaptor) BruceB_10 (head-to-tail stopper) BruceB_11 BruceB_12 (tail terminator) BruceB_13 (major tail protein) BruceB_14 (tail assembly chaperone) BruceB_15 (tail assembly chaperone) BruceB_16 (tape measure protein) BruceB_17 (minor tail protein) BruceB_18 (minor tail protein) BruceB_19 (minor tail protein) BruceB_20 (minor tail protein) BruceB_21 (minor tail protein) BruceB_22 (minor tail protein) BruceB_23 BruceB_24 BruceB_25 BruceB_26 BruceB_27 (lysin A) BruceB_28 (lysin B) BruceB_29 (holin) BruceB_30 BruceB_31 BruceB_32 (integrase) BruceB_33 (immunity repressor) BruceB_34 (recombination directionality factor) BruceB_35 BruceB_36 BruceB_37 BruceB_38 (activating signal cointegrator-like protein) BruceB_39 BruceB_40 BruceB_41 BruceB_42 (exonuclease) BruceB_43 (ssDNA binding protein) BruceB_44 BruceB_45 BruceB_46 BruceB_47 BruceB_48 BruceB_49 BruceB_50 BruceB_51 (RuvC-like resolvase) BruceB_52 BruceB_53 BruceB_54 BruceB_55 BruceB_56 BruceB_57 BruceB_58 (MPME1 protein) BruceB_59 BruceB_60 BruceB_61 BruceB_62 (HNH endonuclease) BruceB_63
Submitted Annotation in DNA Master Format	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KX443326
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	42
Pitt Freezer Box Grid#	54
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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