| Detailed Information for Phage Cambiare |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meaghan Duffy |
| Year Found | 2014 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.444282, -79.954022 Map |
| Discovery Notes | Cambiare was discovered outside of William Pitt Union on University of Pittsburgh campus grounds. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 18, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1365 |
| Genome length (bp) | 45161 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCCATGGCAT |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques created by Cambiare are consistently turbid and slightly larger than a pinprick. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR080198 |
| Refseq Number | None yet |
| Published in Paper | Pope WH et al 2015, Genome Announcements: Genome sequences of Mycobacteriophages Luchador and Nerujay |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
