| Detailed Information for Phage Chestnut | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Andrew Conboy |
| Year Found | 2015 |
| Location Found | Radnor, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.099299 N, 75.259635 W Map |
| Discovery Notes | From McCarthy Park Stormwater Retention Basin in Whitemarsh Township, PA. Soil contained decomposing organic material, as well as a high amount of moisture from a nearby pond. From the same soil sample as Conboy phage. |
| Naming Notes | Chestnut was named after my home institution, Chestnut Hill College, in Philadelphia, Pennsylvania. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3314 |
| Genome length (bp) | 15556 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewArby Arielagos Azathoth Blair CGermain Chestnut Copper Courtney3 Decurro Dewayne Elkhorn Guntur Hunnie Inspire2 Jessica Kels KylieMac Laila Link Lore LouisXIV Maggie Mariposa Massimo Moloch Muttlie Prospero Ronnie Sandman Saphira SerialPhiller Seume Sourignavong StewieGriff Stratus Swenson Taj14 TinoCrisci Toulouse TymAbreu Yank |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Phage tends to form mostly large plaques, with the occasional smaller sized plaque. Optimal temperature is between room temperature and 30 degrees Celsius. Does not grow at 37 degrees Celsius. Grows just as well without any calcium (at optimal temperature) as it does with 25 mM CaCl2 concentration. |
| Morphotype | Siphoviridae |
| Number of Genes | 26 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewChestnut_1 Chestnut_2 Chestnut_3 Chestnut_4 Chestnut_5 Chestnut_6 Chestnut_7 Chestnut_8 Chestnut_9 Chestnut_10 Chestnut_11 Chestnut_12 Chestnut_13 Chestnut_14 Chestnut_15 Chestnut_16 Chestnut_17 Chestnut_18 Chestnut_19 Chestnut_20 Chestnut_26 Chestnut_22 Chestnut_23 Chestnut_24 Chestnut_25 Chestnut_27 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY434670 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Cabrini College |
| SEA Lysate Titer | 1.26E10 |
| Date of SEA Lysate Titering | Jul 15, 2015 |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | B10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
