| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Samuel Garmoe & Angelina Zater |
| Year Found | 2019 |
| Location Found | Baltimore, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.25366 N, 76.71331 W Map |
| Discovery Notes | from an ivy garden. sample was dry and grainy without visible insects present |
| Naming Notes | named after a combo of our dogs' names, Cricket and Koda |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2667 |
| Genome length (bp) | 57623 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI4 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Cricko can reproduce via the lysogenic cycle |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are turbid and approximately 1.8 mm in diameter on average |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT310854 |
| Refseq Number | None yet |
| Published in Paper | Park et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces Bacteriophages Annihilus, TonyStarch, Thiqqums, CricKo, ClubPenguin, RosaAsantewaa, and PherryCruz |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.5e9 |
| Pitt Freezer Box# |
101 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
