| Detailed Information for Phage Darionha |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Amber Abram |
| Year Found | 2017 |
| Location Found | Detroit, MI USA |
| Finding Institution | University of Detroit Mercy |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.417159 N, 83.139801 W Map |
| Discovery Notes | Sample was collected from beneath a tree in front of Shiple Hall at the University of Detroit Mercy. Soil sample collected from 2 inches below the surface in 50 degree and sunny weather. |
| Naming Notes | I have chosen to name this phage in honor of my dearly departed sister Darionha Tyler. Although her body has departed, her name will live on, both in my heart and in this database. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 29, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3342 |
| Genome length (bp) | 41901 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Detroit Mercy |
| Annotation Status | In GenBank |
| Plaque Notes | Phage is lytic. Plaques were about .05 centimeters in diameter and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524493 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
G1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
