Streptomyces phage Ditters
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Detailed Information for Phage Ditters
Discovery Information
Isolation HostStreptomyces lividans NRRL B-12275
Found ByColeman Pennington
Year Found2025
Location FoundDenton, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.177286 N, 97.12708 W Map
Discovery NotesSoil was collected on 08/19/2025 at approximately 6:00-6:30 pm at Pilot Knoll Park, with the approximate coordinates of (33.1061993, -97.0705071). The temperature was approximately 91°F with little to no cloud coverage. Soil was collected at first with a biodegradable plastic spoon, then with a steel pocket knife, then with a nail filer of a multipurpose pocket tool. The excavation site left behind a circular hole, measuring ~3 1/8 inches in circumference and ~1 inch deep. Soil was then scooped by the biodegradable plastic spoon into a plastic bag. About 1/6 of the bag was filled before dropping the spoon into the bag. Soil was very dry and consisted of mainly clay and gravel, with minimal organic material.
Naming NotesDitters was named after the classical violin player Carl Ditters von Dittersdorf, where many of his concertos were transposed into double bass concertos.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 22, 2025
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NextSeq 1000
Read TypeSingle-end reads
Read Length100 bp
Approximate Shotgun Coverage202
Genome length (bp)44012
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCCCT
GC Content60.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBI
SubclusterBI2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusBeing Annotated (Expected completion by 5/1/2026)
Plaque NotesThe average diameter for the highest dilution was about 1.3mm. It was clear and circular.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.1*10^11 pfu/mL
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload