| Detailed Information for Phage Doxi13 |
| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Becca Elrod, Jessica Schafer |
| Year Found | 2021 |
| Location Found | Laurel, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.15222 N, 76.96722 W Map |
| Discovery Notes | Soil collected on 9/15/2021. Dry/sandy soil type. Foggy/humid weather. |
| Naming Notes | We like dachshunds and the number 13. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 25, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 10298 |
| Genome length (bp) | 43696 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Virulent |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, symmetrical, smooth edges, ~1.53 mm diameter (n = 5). |
| Morphotype | Siphoviridae |
| Number of Genes | 60 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970617 |
| Refseq Number | None yet |
| Published in Paper | Jin et al 2024, Microbiology Resource Announcements: Genome characterization of BI2 subcluster Streptomyces scabiei bacteriophages GoblinVoyage and Doxi13 |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.3 x 10^8 pfr/ml |
| Pitt Freezer Box# |
132 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
