Arthrobacter phage ElectricPheel
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| Detailed Information for Phage ElectricPheel | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Hannah Kang |
| Year Found | 2024 |
| Location Found | Santa Monica, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.017106 N, 118.445152 W Map |
| Discovery Notes | Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. Sample type: Moist Sample description: Urban, 50 feet from the closes building, not much foot traffic, borders Centinela Ave., parking lot cement within 20 feet of collection site, moisture in air and grass watered often, tree right above the collection site, area was not sloped Depth from which sample was taken: 10cm Air temperature if sample collected outdoors: 20℉ Additional information of interest or note: Airport park with dog park with dog park within 10 feet of collection point. Plane hangar next to the collection point. Flea market once every two weeks in the parking lot next to the park. AL Quarter and Lab section: F24-1A Instructor: Dr. Tejas Bouklas and Dr. Daniel Velez-Ramirez Teaching Assistant: Praneel Bonthala Undergraduate Assistant(s): Cindy Vo and Andreas Krumbein Team #1 Name: Biohazard Baddies Team #1 Members: Noah Graham, Hannah Kang, Sophie Nuckowski, Sarah Bonver Team #2 Name: Phage Phreaks Team #2 Members: Brooklyn Burgess, Diego Sahagun, Kevin Zhang, Sara Piao Team #3 Name: Vicious Viruses Team #3 Members: Pranava Jana, David Mastro, Alondra Echeverria, Usha Namburu Team #4 Name: Red Hawt Chili Phages Team #4 Members: Alex Baugh, Matilde Iasi, Taniya Mathew, Noah Reynolds |
| Naming Notes | After the song Electric Feel - MGMT (listen to it, it’s a banger) |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 27623 |
| Genome length (bp) | 15597 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 59.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewArby Arielagos Azathoth Blair CGermain Chestnut Copper Courtney3 Decurro Dewayne ElectricPheel Elkhorn Guntur Hunnie Inspire2 Jessica Kels KylieMac Laila Link Lore LouisXIV Maggie Mariposa Massimo Moloch Muttlie Prospero Ronnie Sandman Saphira SerialPhiller Seume Sourignavong StewieGriff Stratus Swenson Taj14 TinoCrisci Toulouse TymAbreu Yank |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology: clear, defined edges Average size (and size range) diameter in mm: 2mm |
| Morphotype | Siphoviridae |
| Number of Genes | 27 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewElectricPheel_1 ElectricPheel_2 ElectricPheel_3 ElectricPheel_4 ElectricPheel_5 ElectricPheel_6 ElectricPheel_7 ElectricPheel_8 ElectricPheel_9 ElectricPheel_10 ElectricPheel_11 ElectricPheel_12 ElectricPheel_13 ElectricPheel_14 ElectricPheel_15 ElectricPheel_16 ElectricPheel_17 ElectricPheel_18 ElectricPheel_19 ElectricPheel_20 ElectricPheel_21 ElectricPheel_22 ElectricPheel_23 ElectricPheel_24 ElectricPheel_25 ElectricPheel_26 ElectricPheel_27 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
