| Detailed Information for Phage Espio | |
| Discovery Information | |
| Isolation Host | Streptomyces lividans NRRL B-12275 |
| Found By | Blu Cedillos-Obregon |
| Year Found | 2025 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.210833 N, 97.150833 W Map |
| Discovery Notes | On the campus of Univeristy of North Texas, near a tree by Chilton Hall. The outside weather was 50% humid with the temperature being 91 F. The date and time it was collected was August 18th, 2025, at 9:02 PM. The soil was slightly damp due to the soil collected being collected at about half an inch under the dry soil. |
| Naming Notes | The name was chosen from the name of a fictional chameleon. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 160 |
| Genome length (bp) | 56447 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 59.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BI |
| Subcluster | BI1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The lysogeny results were positive. Later tests to see if phages could infect the lysogen were inconclusive. |
| Other Cluster Members |
Click to ViewCherryBlossom (BI1) DrGrey (BI1) Esketit (BI1) Espio (BI1) FidgetOrca (BI1) FrodoSwaggins (BI1) GirlPower (BI1) HazuAndZazu (BI1) Hoshi (BI1) IceWarrior (BI1) Indigenous (BI1) Jaylociraptor (BI1) Madamato (BI1) Maya (BI1) Meibysrarus (BI1) Namo (BI1) OlympicHelado (BI1) Popy (BI1) Rima (BI1) Soshi (BI1) Spectropatronm (BI1) TaidaOne (BI1) TieDye (BI1) TonyStarch (BI1) Wigberry (BI1) Annihilus (BI2) Bilo (BI2) Ditters (BI2) Doxi13 (BI2) GoblinVoyage (BI2) HotFries (BI2) Monse (BI2) Moozy (BI2) PherryCruz (BI2) RavenPuff (BI2) RosaAsantewaa (BI2) Sangak (BI2) Scap1 (BI2) SheRa (BI2) LibertyBell (BI3) CricKo (BI4) FromIndy (BI4) Miek (BI4) Rainydai (BI4) Regigigas (BI4) SendItCS (BI4) Thiqqums (BI4) Bing (BI5) Kardashian (BI6) ClubPenguin (BI7) |
| Annotating Institution | University of North Texas |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaques were around 2mm in size, with the majority of the plaques being clear and semi circular. |
| Number of Genes | 85 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEspio_1 Espio_2 Espio_3 Espio_4 Espio_5 Espio_6 Espio_7 Espio_8 Espio_9 Espio_10 Espio_11 Espio_12 Espio_13 Espio_14 Espio_15 Espio_16 Espio_17 Espio_18 Espio_19 Espio_20 Espio_21 Espio_22 Espio_23 Espio_24 Espio_25 Espio_26 Espio_27 Espio_28 Espio_29 Espio_30 Espio_31 Espio_32 Espio_33 Espio_34 Espio_35 Espio_36 Espio_37 Espio_38 Espio_39 Espio_40 Espio_41 Espio_42 Espio_43 Espio_44 Espio_45 Espio_46 Espio_47 Espio_48 Espio_49 Espio_50 Espio_51 Espio_52 Espio_53 Espio_54 Espio_55 Espio_56 Espio_57 Espio_58 Espio_59 Espio_60 Espio_61 Espio_62 Espio_63 Espio_64 Espio_65 Espio_66 Espio_67 Espio_68 — tRNA Espio_69 Espio_70 Espio_71 Espio_72 Espio_73 Espio_74 Espio_75 Espio_76 Espio_77 Espio_78 Espio_79 Espio_80 Espio_81 Espio_82 Espio_83 Espio_84 Espio_85 Espio_86 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
