| Detailed Information for Phage FeliMaine |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jindanuch Maneekul |
| Year Found | 2024 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.445352 N, 79.953004 W Map |
| Discovery Notes | I got the soil sample from under a tree near the Cathedral of learning on 3/25/24. Temp 63F, 20% humidity. |
| Naming Notes | FeliMaine simply means "Maine the cat". Feli is from Felidae, the cat family. Maine is my orange tabby who got to Pittsburgh in 2023. Similarly, phage FeliMaine is the first phage I brought to the Hatfull lab in the following months. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 19266 |
| Genome length (bp) | 64981 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | Being Annotated (Expected completion by 11/14/2024) |
| Plaque Notes | The plaques are round, very tiny (<0.5mm), and clear. However, it can become cloudy when spotted or plated with a very high concentration. The plaques take 2 days to appear on agar at 37C. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
1x10^12 pfu/mL |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
