| Detailed Information for Phage FeliMaine | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jindanuch Maneekul |
| Year Found | 2024 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.445352 N, 79.953004 W Map |
| Discovery Notes | I got the soil sample from under a tree near the Cathedral of learning on 3/25/24. Temp 63F, 20% humidity. |
| Naming Notes | FeliMaine simply means "Maine the cat". Feli is from Felidae, the cat family. Maine is my orange tabby who got to Pittsburgh in 2023. Similarly, phage FeliMaine is the first phage I brought to the Hatfull lab in the following months. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 19266 |
| Genome length (bp) | 64981 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | GCGCGCAGCGC |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | S |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | Overdue (Expected completion by 11/14/2024) |
| Plaque Notes | The plaques are round, very tiny (<0.5mm), and clear. However, it can become cloudy when spotted or plated with a very high concentration. The plaques take 2 days to appear on agar at 37C. |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewFeliMaine_1 FeliMaine_2 FeliMaine_3 FeliMaine_4 FeliMaine_5 FeliMaine_6 FeliMaine_7 FeliMaine_8 FeliMaine_9 FeliMaine_10 FeliMaine_11 FeliMaine_12 FeliMaine_13 FeliMaine_14 FeliMaine_15 FeliMaine_16 FeliMaine_17 FeliMaine_18 FeliMaine_19 FeliMaine_20 FeliMaine_21 FeliMaine_22 FeliMaine_23 FeliMaine_24 FeliMaine_25 FeliMaine_26 FeliMaine_27 FeliMaine_28 FeliMaine_29 FeliMaine_30 FeliMaine_31 FeliMaine_32 FeliMaine_33 FeliMaine_34 FeliMaine_35 FeliMaine_36 FeliMaine_37 FeliMaine_38 FeliMaine_39 FeliMaine_40 FeliMaine_41 FeliMaine_42 FeliMaine_43 FeliMaine_44 FeliMaine_45 FeliMaine_46 FeliMaine_47 FeliMaine_48 FeliMaine_49 FeliMaine_50 FeliMaine_51 FeliMaine_52 FeliMaine_53 FeliMaine_54 FeliMaine_55 FeliMaine_56 FeliMaine_57 FeliMaine_58 FeliMaine_59 FeliMaine_60 FeliMaine_61 FeliMaine_62 FeliMaine_63 FeliMaine_64 FeliMaine_65 FeliMaine_66 FeliMaine_67 FeliMaine_68 FeliMaine_69 FeliMaine_70 FeliMaine_71 FeliMaine_72 FeliMaine_73 FeliMaine_74 FeliMaine_75 FeliMaine_76 FeliMaine_77 FeliMaine_78 FeliMaine_79 FeliMaine_80 FeliMaine_81 FeliMaine_82 FeliMaine_83 FeliMaine_84 FeliMaine_85 FeliMaine_86 FeliMaine_87 FeliMaine_88 FeliMaine_89 FeliMaine_90 FeliMaine_91 FeliMaine_92 FeliMaine_93 FeliMaine_94 FeliMaine_95 FeliMaine_96 FeliMaine_97 FeliMaine_98 FeliMaine_99 FeliMaine_100 FeliMaine_101 FeliMaine_102 FeliMaine_103 FeliMaine_104 FeliMaine_105 FeliMaine_106 FeliMaine_107 FeliMaine_108 FeliMaine_109 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1x10^12 pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
