The Actinobacteriophage Database

Mycobacterium phage FlagStaff

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Detailed Information for Phage FlagStaff
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Chandler Mitchell
Year Found	2014
Location Found	Pittsburgh, PA USA
Finding Institution	University of Pittsburgh
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	40.399 N, 79.946 W Map
Discovery Notes	Obtained from Schenley park, on Flagstaff hill.
Naming Notes	Named after location obtained
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 19, 2015
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	4457
Genome length (bp)	44576
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CCCATGGCAT
GC Content	68.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	G
Subcluster	G2
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Angel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) AzulaCat (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Maliketh (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Remy19 (G1) Renaissance (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TomBrady (G1) Wendigo (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) Pace1224 (G2) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Lysogenic Pinpoint plaques that are very hazy.
Morphotype	Siphoviridae
Number of Genes	65
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View FlagStaff_1 (terminase small subunit) FlagStaff_2 (terminase large subunit) FlagStaff_3 (portal protein) FlagStaff_4 (capsid maturation protease) FlagStaff_5 FlagStaff_6 FlagStaff_7 (major capsid protein) FlagStaff_8 FlagStaff_9 (head-to-tail adaptor) FlagStaff_10 (head-to-tail stopper) FlagStaff_11 FlagStaff_12 (tail terminator) FlagStaff_13 (major tail subunit) FlagStaff_14 (tail assembly chaperone) FlagStaff_15 (tail assembly chaperone) FlagStaff_16 (tape measure protein) FlagStaff_17 (minor tail protein) FlagStaff_18 (minor tail protein) FlagStaff_19 (minor tail protein) FlagStaff_20 (minor tail protein) FlagStaff_21 (minor tail protein) FlagStaff_22 (minor tail protein) FlagStaff_23 FlagStaff_24 FlagStaff_25 FlagStaff_26 (lysin A) FlagStaff_27 (lysin B) FlagStaff_28 (holin) FlagStaff_29 FlagStaff_30 FlagStaff_31 FlagStaff_32 FlagStaff_33 FlagStaff_34 FlagStaff_35 FlagStaff_36 FlagStaff_37 FlagStaff_38 FlagStaff_39 FlagStaff_40 FlagStaff_41 FlagStaff_42 (RecE) FlagStaff_43 (RecT) FlagStaff_44 FlagStaff_45 FlagStaff_46 FlagStaff_47 FlagStaff_48 FlagStaff_49 (RuvC) FlagStaff_50 FlagStaff_51 FlagStaff_52 FlagStaff_53 FlagStaff_54 FlagStaff_55 FlagStaff_56 FlagStaff_57 FlagStaff_58 FlagStaff_59 FlagStaff_60 FlagStaff_61 FlagStaff_62 FlagStaff_63 FlagStaff_64 (HNH endonuclease) FlagStaff_65
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KR080197
Refseq Number	None yet
Published in Paper	Pope WH et al 2015, Genome Announcements: Genome Sequences of Cluster G Mycobacteriophages Cambiare, FlagStaff, and MOOREtheMARYer.
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	1
Pitt Freezer Box Grid#	E1
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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