Mycobacterium phage Frosty24
Frosty24 Plaque Picture
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Detailed Information for Phage Frosty24
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByReshard McElrath
Year Found2011
Location FoundAtlanta, GA USA
Finding InstitutionMorehouse College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.747650 N, 84.415900 W Map
Discovery NotesArea that contains plants beside a greenhouse. Soil was taken from the surface.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 26, 2012
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent
Approximate Shotgun Coverage85
Genome length (bp)41901
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members

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Angel (G1)

Annihilator (G1)

Aroostook (G1)

Avrafan (G1)

AzulaCat (G1)

Barkley26 (G1)

BPs (G1)

BQuat (G1)

BruceB (G1)

Camri (G1)

CassieYates (G1)

Cedasite (G1)

Chance64 (G1)

Cherrybomb426 (G1)

CLED96 (G1)

Coleslaw (G1)

Crespo (G1)

Darionha (G1)

DMoney (G1)

ECartman (G1)

Frosty24 (G1)

Gideon (G1)

GoldenAsh (G1)

Gomashi (G1)

Grizzly (G1)

Halo (G1)

Hope (G1)

Hotshotbaby7 (G1)

Jane (G1)

Jolene (G1)

Jonghyun (G1)

JorRay (G1)

Kareem (G1)

Kasen3 (G1)

Liefie (G1)

LouisV14 (G1)

Maliketh (G1)

Marmie (G1)

Mowgli (G1)

OctaviousRex (G1)

Olga (G1)

Paito (G1)

Peeb (G1)

Periodt (G1)

Phish (G1)

Phreak (G1)

PinkYoshi (G1)

Plagueis (G1)

Rabbs (G1)

Remy19 (G1)

Renaissance (G1)

Schiebel (G1)

ShaboiShabazz (G1)

Sizemore (G1)

Sneeze (G1)

Sweets (G1)

Taheera (G1)

Terror (G1)

TomBrady (G1)

Wendigo (G1)

ZoMa (G1)

Zombie (G1)

Avocado (G2)

Cambiare (G2)

FlagStaff (G2)

Pace1224 (G2)

MOOREtheMARYer (G3)

Pinnie (G3)

Jolie2 (G4)

Lemuria (G4)

Mercurio (G4)

Antsirabe (G5)

Stargaze (G5)
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesExtremely lysed. Small plaque.
MorphotypeSiphoviridae
Number of Genes63
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Frosty24_1
(terminase small subunit)

Frosty24_2
(terminase large subunit)

Frosty24_3
(portal protein)

Frosty24_4
(capsid maturation protease)

Frosty24_5

Frosty24_6
(scaffolding protein)

Frosty24_7
(major capsid protein)

Frosty24_8
(head-to-tail connector protein)

Frosty24_9
(head-to-tail adaptor)

Frosty24_10
(head-to-tail stopper)

Frosty24_11

Frosty24_12
(tail terminator)

Frosty24_13
(major tail protein)

Frosty24_14
(tail assembly chaperone)

Frosty24_15
(tail assembly chaperone)

Frosty24_16
(tape measure protein)

Frosty24_17

Frosty24_18
(minor tail protein)

Frosty24_19

Frosty24_20
(minor tail protein)

Frosty24_21
(minor tail protein)

Frosty24_22
(minor tail protein)

Frosty24_23

Frosty24_24

Frosty24_25

Frosty24_26

Frosty24_27
(lysin A)

Frosty24_28
(lysin B)

Frosty24_29
(holin)

Frosty24_30

Frosty24_31

Frosty24_32
(integrase)

Frosty24_33
(immunity repressor)

Frosty24_34
(excise)

Frosty24_35

Frosty24_36

Frosty24_37

Frosty24_38
(ASC-1-like domain protein)

Frosty24_39

Frosty24_40

Frosty24_41

Frosty24_42
(exonuclease)

Frosty24_43
(ssDNA binding protein)

Frosty24_44

Frosty24_45

Frosty24_46

Frosty24_47

Frosty24_48

Frosty24_49

Frosty24_50

Frosty24_51
(RuvC-like resolvase)

Frosty24_52

Frosty24_53

Frosty24_54

Frosty24_55

Frosty24_56

Frosty24_57

Frosty24_58

Frosty24_59

Frosty24_60

Frosty24_61

Frosty24_62
(HNH endonuclease)

Frosty24_63
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT355474
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator Dr. Piester
Pitt Freezer Box# 26
Pitt Freezer Box Grid# 24
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload