| Detailed Information for Phage GoblinVoyage | |
| Discovery Information | |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Annie Tang and Hanna Jin |
| Year Found | 2023 |
| Location Found | Golansville, VA United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.980611 N, 77.491896 W Map |
| Discovery Notes | Soil was dry and dusty on surface, slighlty below soil surface contained slightly dry soil with larger clumps. Soil still mostly dry, light to medium brown in coloration, very few debris in sample. Sample taken from the bottom of a large deciduous tree. Temperature on 9/4 when sample was taken was around 82 degrees fahrenheit. Humidity that day was between 65% to 75%. Soil sample was processed on 9/19. |
| Naming Notes | As the phage was found from a soil sample from Golansville, VA, we decided to stick with a name that started with a "G" and "V", resulting in "GoblinVoyage". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1166 |
| Genome length (bp) | 43540 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | No lysogens noted after over a week of incubation |
| Other Cluster Members |
Click to ViewCherryBlossom (BI1) DrGrey (BI1) Esketit (BI1) FidgetOrca (BI1) FrodoSwaggins (BI1) GirlPower (BI1) HazuAndZazu (BI1) Hoshi (BI1) IceWarrior (BI1) Indigenous (BI1) Jaylociraptor (BI1) Madamato (BI1) Maya (BI1) Meibysrarus (BI1) Namo (BI1) OlympicHelado (BI1) Popy (BI1) Rima (BI1) Soshi (BI1) Spectropatronm (BI1) TaidaOne (BI1) TieDye (BI1) TonyStarch (BI1) Annihilus (BI2) Bilo (BI2) Doxi13 (BI2) GoblinVoyage (BI2) HotFries (BI2) Moozy (BI2) PherryCruz (BI2) RavenPuff (BI2) RosaAsantewaa (BI2) Scap1 (BI2) SheRa (BI2) LibertyBell (BI3) CricKo (BI4) FromIndy (BI4) Miek (BI4) Rainydai (BI4) Regigigas (BI4) SendItCS (BI4) Thiqqums (BI4) Bing (BI5) Kardashian (BI6) ClubPenguin (BI7) |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | On Streptomyces scabiei and Streptomyces mirabilis, plaques were between 0.5 and 3mm in diameter. S. scabiei plaques were turbid and S. mirabilis plaques were clear. Both had clean margins. |
| Morphotype | Siphoviridae |
| Number of Genes | 59 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGoblinVoyage_1 GoblinVoyage_2 GoblinVoyage_3 GoblinVoyage_4 GoblinVoyage_5 GoblinVoyage_6 GoblinVoyage_7 GoblinVoyage_8 GoblinVoyage_9 GoblinVoyage_10 GoblinVoyage_11 GoblinVoyage_12 GoblinVoyage_13 GoblinVoyage_14 GoblinVoyage_15 GoblinVoyage_16 GoblinVoyage_17 GoblinVoyage_18 GoblinVoyage_19 GoblinVoyage_20 GoblinVoyage_21 GoblinVoyage_22 GoblinVoyage_23 GoblinVoyage_24 GoblinVoyage_25 GoblinVoyage_26 GoblinVoyage_27 GoblinVoyage_28 GoblinVoyage_29 GoblinVoyage_30 GoblinVoyage_31 GoblinVoyage_32 GoblinVoyage_33 GoblinVoyage_34 GoblinVoyage_35 GoblinVoyage_36 GoblinVoyage_37 GoblinVoyage_38 GoblinVoyage_39 GoblinVoyage_40 GoblinVoyage_41 GoblinVoyage_42 GoblinVoyage_43 GoblinVoyage_44 GoblinVoyage_45 GoblinVoyage_46 GoblinVoyage_47 GoblinVoyage_48 GoblinVoyage_49 GoblinVoyage_50 GoblinVoyage_51 GoblinVoyage_52 GoblinVoyage_53 GoblinVoyage_54 GoblinVoyage_55 GoblinVoyage_56 GoblinVoyage_57 GoblinVoyage_58 GoblinVoyage_59 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP725412 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 171 |
| Pitt Freezer Box Grid# | C2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
